{"title":"Contract Projects","description":"\u003cstyle\u003e\n  #shopify-section-collection-template \u003e div \u003e div:nth-child(4) {display: none;}\n\u003c\/style\u003e\n\u003cdiv class=\"rte\"\u003e\n\u003ch2\u003eWe Help Find the Genes that Matter to You!\u003c\/h2\u003e\n\u003cp\u003eEnd-to-end or modular service options for life science researchers seeking to uncover functional genes important for phenotypic responses, disease progression, therapeutic responses, signaling pathways, or genetic requirements for drug mechanism of action or resistance.\u003c\/p\u003e\n\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eCellecta’s Contract Projects Group supports biotech, pharma, government, and academic labs looking to identify gene function, mechanisms of action, drug response markers, or disease drivers with fully customized solutions including CRISPR and RNAi screening, perturbation-based single-cell transcriptomics, and engineered cell line development. We provide quality data on time and within budget to research laboratories—saving you the cost and burden of building internal screening infrastructure.\u003c\/p\u003e\n\u003ch3\u003eCustom Research Solutions\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eFull-Service CRISPR \u0026amp; shRNA Screens\u003c\/li\u003e\n\u003cli\u003eComprehensive Cell Line Engineering\u003c\/li\u003e\n\u003cli\u003ePerturb-seq and Single-Cell CRISPR Screens\u003c\/li\u003e\n\u003cli\u003eCustom Library Development \u0026amp; Sequencing\u003c\/li\u003e\n\u003cli\u003eImmune Profiling \u0026amp; Repertoire Sequencing\u003c\/li\u003e\n\u003cli\u003eBioinformatics Analysis Capabilities\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eThe Cellecta Advantage\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDeep Functional Genomics and Cell Biology Expertise\u003c\/li\u003e\n\u003cli\u003eCollaborative Project Scoping to Define Assay Design and End Goals\u003c\/li\u003e\n\u003cli\u003eFlexible Project-Based Engagement Models\u003c\/li\u003e\n\u003cli\u003eResults-Focused Deliverables\u003c\/li\u003e\n\u003cli\u003eProven Workflows Established in House by Experienced Scientists\u003c\/li\u003e\n\u003cli\u003ePublished Outcomes \u0026amp; Peer Credibility\u003c\/li\u003e\n\u003cli\u003eTransparent Timelines \u0026amp; Pricing\u003c\/li\u003e\n\u003cli\u003eConfidentiality\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eRecord of Success\u003c\/h3\u003e\n\u003ch4\u003eSample Typical Projects\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003eCRISPR drug response or resistance screens with genome-wide or targeted sgRNA libraries\u003c\/li\u003e\n\u003cli\u003eMaking and screening UTR-reporter libraries for translational control regions\u003c\/li\u003e\n\u003cli\u003ePerturb-seq screens using the most appropriate scRNA-seq platform\u003c\/li\u003e\n\u003cli\u003eCRISPR screens with non-Cas9 systems such as dCas9-VPH, dCas9-KRAB, Cas13d, etc.\u003c\/li\u003e\n\u003cli\u003eInducible knock-in\/knock-out cell line development\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eGet Started\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cellecta.com\/pages\/crispr-rnai-screening-services-quote-request\"\u003eSchedule a Technical Consultation\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eor\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eRequest a project quote using one of the links below:\u003c\/p\u003e\n\u003c\/div\u003e","products":[{"product_id":"crispr-rnai-screening-services","title":"CRISPR\/RNAi Screening Services","description":"\u003cp\u003e\u003ca href=\"\/pages\/crispr-rnai-screening-services-quote-request\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e  Get A Quote  \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eLet Cellecta put its years of RNAi and CRISPR screening expertise to work for you. We offer full-scale genetic screening with pooled lentiviral sgRNA and shRNA libraries to identify the gene targets driving biological responses in your cell lines or model systems of interest. We will run the CRISPR or RNAi screen so you can\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIdentify genes essential for growth and proliferation\u003c\/li\u003e\n\u003cli\u003eFind genes responsible for resistance or sensitivity to compounds or treatments\u003c\/li\u003e\n\u003cli\u003eLook for synthetic lethal interactions with known genetic lesions\u003c\/li\u003e\n\u003cli\u003eElucidate the mechanism of action or target pathway for a compound or drug\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eContact us to discuss your specific CRISPR or shRNA screening needs. More  details below \u0026gt;\u003c\/p\u003e\n\u003c!-- split --\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/crispr-rnai-screening-services.png\" class=\"lightbox\" data-fancybox=\"\"\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/crispr-rnai-screening-services.png\" alt=\"CRISPR\/RNAi Screening Services\" class=\"image-right lazyloaded\"\u003e\u003c\/a\u003eCellecta provides full CRISPR and RNAi screening services to help you identify the genes or genetic elements functionally regulating a biological response. We perform all steps for comprehensive pooled screens with your cell models. Tell us about the cells, phenotype, and function of the genes you are interested in identifying. Then, we will run the screen and provide you a report with comprehensive data indicating which gene(s)--when disrupted or perturbed by CRISPR sgRNA or RNAi shRNA--affect the response of interest. Cellecta can \u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRun genome-wide or targeted CRISPR knockout, CRISPRa, CRISPRi, and RNAi screens to meet your specific goals\u003c\/li\u003e\n\u003cli\u003eScreen one of our human or mouse genome-wide pooled libraries, or a custom library of any size, for any species\u003c\/li\u003e\n\u003cli\u003eFor CRISPR screens, we make and test Cas9-expressing cell lines, which can be provided to you for future studies.\u003c\/li\u003e\n\u003cli\u003eChoose from a variety of FACS-based reporter and antibody selection options, in addition to more typical viability screens\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eWe can provide all the services required to run most screens, including testing cell responses (e.g., kill curves, antibiotic sensitivity, etc.), cell engineering to make reporter cell lines and Cas9\/dCas9-expressing lines, library construction and packaging, next-generation sequencing, and alignment to identify hits, and post-screening downstream analysis. Provide some details when you request a quote via the link above, and we will be happy to discuss your project with you.\u003c\/p\u003e\n\u003cp\u003eMore information on pooled genetic screens available \u003ca rel=\"noopener\" title=\"Cellecta information on functional genetic screens with pooled shrna or sgrna libraries\" href=\"https:\/\/cellecta.com\/pages\/genetic-screens-with-pooled-shrna-or-sgrna-libraries\" target=\"_blank\"\u003ehere \u0026gt; \u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eCellecta also offers single-cell CRISPR screening services. Learn more \u003ca rel=\"noopener\" title=\"Cellecta offers single-cell CRISPR screening services Perturb-Seq CROP-Seq CRISPR-Seq\" href=\"https:\/\/cellecta.com\/products\/single-cell-crispr-screening-service\" target=\"_blank\"\u003ehere \u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eLearn more about our CRISPR \u0026amp; RNAi Functional Genomic Capabilities \u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-Brochure_8.5x11-r6_WEB.pdf?v=1760647471\" target=\"_blank\"\u003ehere\u0026gt;\u003c\/a\u003e \u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-Brochure_A4-r6-WEB.pdf?v=1760647471\" target=\"_blank\"\u003e(A4)\u003c\/a\u003e\u003c\/p\u003e","brand":"Genetic Screens","offers":[{"title":"Default Title","offer_id":17856624394330,"sku":"CRG","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/products\/Genetic-Screening-Simple.jpg?v=1567626674"},{"product_id":"drivermap-targeted-rna-sequencing-service","title":"DriverMap EXP Targeted RNA Sequencing Expression Profiling Service","description":"\u003cp\u003e\u003ca href=\"\/pages\/drivermap-rna-sequencing-service-quote-request\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e  Get A Quote  \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003eDriverMap Targeted RNA Expression Profiling Service\u003c\/strong\u003e measures the expression level of approximately 19,000 protein-coding genes in the human genome or almost 5000 mouse genes from just a few nanograms of total RNA.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eStarting material of a few ng of total RNA from blood--\u003ca href=\"\/collections\/drivermap-targeted-rna-sequencing\/products\/drivermap-human-genome-wide-expression-profiling-kit-v2?variant=17567338561626\" target=\"_blank\" title=\"DriverMap EXP Dried Blood Microsampling Kit\" rel=\"noopener\"\u003eeven from dried blood\u003c\/a\u003e--, tissue, cells or cell lysate, FFPE samples\u003c\/li\u003e\n\u003cli\u003eMore sensitive and better dynamic range than standard RNA sequencing\u003c\/li\u003e\n\u003cli\u003eNormalized quantitative expression levels for each gene target provided, with further downstream analysis available from our in-house bioinformatics team\u003c\/li\u003e\n\u003cli\u003eRapid, 1-month turnaround\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eObtain ultra-sensitive digital expression profiling data for all 19,000 protein-coding genes in the human genome or some 5000 mouse genes with our DriverMap Targeted RNA Sequencing platform.\u003c\/p\u003e\n\u003cp\u003eDownload \u003ca rel=\"noopener\" title=\"Cellecta DriverMap v3 human whole genome EXP assay gene list\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DriverMap_v3_genelist-v1.pdf?v=1731082811\" target=\"_blank\"\u003eHuman genome-wide gene list \u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eDownload \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Cellecta-GENE-List-DriverMap-Mouse-181031.xlsx?v=1562810999\" title=\"Cellecta DriverMap Mouse Gene List\" target=\"_blank\"\u003eMouse gene list \u0026gt;\u003c\/a\u003e\u003c\/p\u003e\n\u003c!-- split --\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DriverMap-Excel-sheet-sample-data-1-1024x435_f23c9220-2c72-4c38-b0ad-00fa7450530c.gif?26963\" class=\"lightbox\" data-fancybox=\"\"\u003e \u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DriverMap-Excel-sheet-sample-data-1-1024x435_f23c9220-2c72-4c38-b0ad-00fa7450530c.gif?26963\" alt=\"DriverMap RNA-Sequencing Service\" class=\"image-right lazyloaded\"\u003e\u003c\/a\u003eCellecta’s DriverMap™ service is a novel, comprehensive end-to-end, whole-genome targeted expression profiling solution intended for research applications.\u003c\/p\u003e\n\u003cp\u003eCellecta’s DriverMap RNA Expression Profiling service includes:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eRNA isolation\u003c\/li\u003e\n\u003cli\u003eSample QC\u003c\/li\u003e\n\u003cli\u003eRT-PCR of samples using the DriverMap primers\u003c\/li\u003e\n\u003cli\u003eNext-generation sequencing (NGS) run\u003c\/li\u003e\n\u003cli\u003eAnalysis to yield gene expression levels\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThe analysis includes deconvolution, quantification, and normalization of the expression levels for each target gene. Further downstream analysis is available at an additional cost.\u003c\/p\u003e\n\u003cp\u003eSince the DriverMap assay is a targeted RT-PCR approach that generates a single amplicon of approximately 200 bases for each target gene, the library only has about 19,000 sequences. This is very different from RNA-Seq, where you essentially sequence the entire transcriptome. As a result, with the DriverMap assay, you can achieve very good depth of coverage and more sensitivity than RNA-Seq, with 5-10 fold fewer reads. We typically obtain about 5 million reads per sample.\u003c\/p\u003e","brand":"Transcriptome Profiling","offers":[{"title":"Default Title","offer_id":17681533239386,"sku":"DMAP","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DMap_EXP_2024_1.png?v=1741296456"},{"product_id":"sequencing-service-for-samples-from-genetic-screens","title":"Sequencing Service for Samples from Genetic Screens and Barcode Libraries","description":"\u003cp\u003e\u003ca href=\"\/pages\/sequencing-service-for-samples-from-genetic-screen-quote-request\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e  Get A Quote  \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eGet results from gene screens with pooled CRISPR, shRNA, and barcode libraries faster by letting Cellecta do the NGS prep, sequencing, and analysis.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSend us cells or DNA and get a report with the number of reads per guide, hairpin, or barcode\u003c\/li\u003e\n\u003cli\u003eRapid turnaround time\u003c\/li\u003e\n\u003cli\u003eAble to sequence all Cellecta libraries and a range of other pooled lentiviral libraries, such as Brunello, GecKO, etc.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eYou provide Cellecta with frozen cell pellets, tissue or genomic DNA and we do the rest!\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.cellecta.com\/order-forms\/Cellecta-NGS-Library-Sample-Form.xlsx\" target=\"_blank\"\u003eDownload Sample Submission Form\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003c!-- split --\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/cellecta-library-ngs-kit-crispr-prsg16-manual.jpg?26965\" class=\"lightbox\" data-fancybox=\"\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cp\u003eIf you are running your own pooled lentiviral genetic screens with CRISPR sgRNA, RNAi hairpin, or using our cell barcode labeling libraries, simply provide harvested cells or genomic DNA for each time point or treatment condition (one sample), and we will provide you with a spreadsheet enumerating the read number for each of the library elements. Our Genetic Screens \u0026amp; Barcode Library Sequencing service...\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eExtracts genomic DNA\u003c\/li\u003e\n\u003cli\u003eAmplifies barcodes or sgRNA\u003c\/li\u003e\n\u003cli\u003ePerforms NGS on the Illumina NextSeq or HiSeq\u003c\/li\u003e\n\u003cli\u003eEnumerates shRNA, sgRNA, or barcode counts from raw sequencing data\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional bioinformatics and pathway analyses including MAGeCK and others are available \u003ca rel=\"noopener noreferrer\" title=\"Email us for information on additional Bioinformatics services from Cellecta\" href=\"mailto:info@cellecta.com\" target=\"_blank\"\u003eupon request\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003eSequencing is provided not only for Cellecta libraries but also similar non-Cellecta CRISPR libraries, such as the GeCKO, Brunello, Brie libraries, etc. We have extensive experience in designing primers and running NGS on a large variety of pooled libraries for various types of genetic screens.\u003c\/p\u003e\n\u003cp\u003eAlternatively, if you prefer to run NGS on the libraries yourself, Cellecta offers a variety of \u003ca title=\"Cellecta Next-Generation Sequencing NGS Analysis Kits for Genetic Screen and Barcode Libraries\" href=\"\/collections\/next-gen-sequencing-crispr-rnai-libraries\" target=\"_blank\"\u003eNGS Analysis kits\u003c\/a\u003e with the primers you need to carry this out.\u003c\/p\u003e","brand":"Sequencing of Screened Samples","offers":[{"title":"Default Title","offer_id":17681776476250,"sku":"CANA","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/LNGS-Prep-Kit-workflow-250402.jpg?v=1784767417"},{"product_id":"drivermap-immune-receptor-profiling-service","title":"DriverMap™ Adaptive Immune Receptor (AIR) TCR-BCR Profiling Service","description":"\u003cp\u003e\u003ca rel=\"noopener\" title=\"Cellecta DriverMap Adaptive Immune Receptor (AIR) Bulk Profiling Service\" href=\"https:\/\/cellecta.wufoo.com\/forms\/drivermap-adaptive-immune-repertoire-profilebulk\/\" target=\"_blank\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e  Get a Quote \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003eDriverMap Adaptive Immune Receptor (AIR) Repertoire Profiling Service\u003c\/strong\u003e from Cellecta \u003cstrong\u003eprovides you with a profile of all TCR and BCR CDR3 or full-length variable regions in blood, cell, or RNA samples.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWith the DriverMap AIR TCR-BCR Profiling Service, you get\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ea larger complement of clonotypes than other similar assays\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003ereproducible and comprehensive coverage from a range of immune sample inputs, including total RNA from whole blood\u003c\/li\u003e\n\u003cli\u003eRapid, 1-month turnaround from sample submission to an extensive analysis report\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch4 style=\"text-align: center;\"\u003e\u003cem\u003eGeneral Information\u003c\/em\u003e\u003c\/h4\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DriverMap-Excel-sheet-sample-data-1-1024x435_f23c9220-2c72-4c38-b0ad-00fa7450530c.gif?26963\" class=\"lightbox\" data-fancybox=\"\"\u003e \u003c\/a\u003eCellecta’s DriverMap™ AIR TCR-BCR assay is designed to specifically amplify\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eonly \u003cstrong\u003efunctional CDR3 RNA\u003c\/strong\u003e molecules from human or mouse TCR and BCR cells, avoiding non-functional pseudogenes with similar structures or \u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003cstrong\u003efull-length variable regions\u003c\/strong\u003e from human RNA molecules' TCR and BCRs\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003e\u003cstrong\u003eT-cell and B-cell receptor genes\u003c\/strong\u003e directly from human \u003cstrong\u003egenomic DNA\u003c\/strong\u003e \u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eThe assay simultaneously amplifies, in a single, multiplex RT-PCR reaction, all TCR and BCR variable regions using a set of 300 experimentally validated PCR primers to yield Illumina-compatible, next-generation sequencing (NGS) libraries.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eWhile the profiling assay can be limited to return data on only the TCR or BCR repertoires, we recommend doing both. Comprehensive analysis of both the TCR and BCR repertoire is a promising strategy for unbiased discovery of predictive and prognostic biomarkers considering that both arms of the adaptive immune system work synergistically for many disease or adaptive immunity stimulus conditions.\u003c\/p\u003e\n\u003cp\u003eAdditionally, samples submitted for profiling using the DriverMap AIR Repertoire Profiling Service can be run, in parallel, through our standard DriverMap Genome-Wide Targeted Gene Expression Profiling assay to generate gene expression profiles that provide phenotypic information for cell typing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDo you have immune samples you would like to profile?\u003c\/strong\u003e Provide us with some information on your samples and your study goals, and we will get back to you with information on how our DriverMap Adaptive Immune Receptor Profiling service can help your reach your experimental goals.\u003c\/p\u003e\n\u003cp\u003eView the \u003ca href=\"https:\/\/www.manula.com\/manuals\/cellecta\/drivermap-air-tcr-bcr-profiling-technology-guide\/v1\/en\/topic\/overview-of-drivermap-air-tcr-bcr-profiling\" target=\"_blank\" title=\"Cellecta DriverMap AIR Technology Guide for an overview of TCR-Seq \/ BCR-Seq for immune receptor profiling\" rel=\"noopener\"\u003eDriverMap AIR Technology Guide\u003c\/a\u003e\u003ca rel=\"noopener\" title=\"Cellecta DriverMap AIR Technology Guide\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DriverMap-EXP-AIR-Brochure-WEB-v7.pdf?v=1738873949\" target=\"_blank\"\u003e\u003c\/a\u003e for an\u003cstrong\u003e introduction to AIR technology\u003c\/strong\u003e and for recommendations on designing AIR profiling experiments.\u003c\/p\u003e\n\u003cp\u003eDownload the \u003ca title=\"DriverMap Adaptive Immune Receptor TCR BCR Profiling FLYER Cellecta\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/TCR-BCR-Profiling-Flier-A4-Web.pdf?v=1738874068\" rel=\"noopener\" target=\"_blank\"\u003eproduct flyer\u003c\/a\u003e for a general overview or learn more about the\u003cstrong\u003e DriverMap AIR Profiling products\u003c\/strong\u003e \u003ca rel=\"noopener\" title=\"Cellecta DriverMap AIR Technology Overview\" href=\"https:\/\/cellecta.com\/collections\/drivermap-adaptive-immune-receptor-air-profiling\" target=\"_blank\"\u003ehere\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eWe have also developed \u003cstrong\u003eRNA Spike-In Controls\u003c\/strong\u003e for use within RNA-based AIR-seq assays. \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/RNA-spike-in-Controls-Flier-d4-Cellecta-WEB.pdf?v=1652818525\" target=\"_blank\"\u003eDownload details here.\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eWatch the \u003cstrong\u003ewebinar \u003c\/strong\u003e\u003ca title=\"Cellecta webinar Immunophenotyping of TCR and BCR Clonotypes\" href=\"https:\/\/www.youtube.com\/watch?v=uVkfftivR7o\" target=\"_blank\"\u003eImmunophenotyping of TCR and BCR Clonotypes\u003c\/a\u003e (26 min) \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePoster\u003c\/strong\u003e: \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/04_2023_TCR_BCR_A0.pdf?v=1682461499\" target=\"_blank\"\u003eAdaptive Immune Receptor Repertoire Profiling for Biomarker Discovery\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Cellecta DriverMap Adaptive Immune Receptor (AIR) Profiling Service for Bulk Samples\" href=\"https:\/\/cellecta.wufoo.com\/forms\/drivermap-adaptive-immune-repertoire-profilebulk\/\" target=\"_blank\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e  Tell us about your samples and request a quote \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"TCR-BCR Repertoire Profiling","offers":[{"title":"Default Title","offer_id":42056839594242,"sku":null,"price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DriverMap_AIR_Assay_Service_slim-No_UMI_c3b5115b-65e0-497a-ad4b-c3be01a42a5b.png?v=1734550684"},{"product_id":"single-cell-crispr-screening-service","title":"Single-Cell CRISPR Screening Service: Perturb-Seq, CRISP-Seq, CROP-Seq etc.","description":"\u003cp\u003e\u003ca rel=\"noopener\" title=\"Cellecta Single-Cell CRISPR Screening Service\" href=\"https:\/\/cellecta.wufoo.com\/forms\/singlecell-crispr-screening-svcs-quote-request\/\" target=\"_blank\"\u003e\u003cbutton class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\" type=\"button\" style=\"width: 100%;\"\u003e  Get A Quote  \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eWith\u003cstrong\u003e Cellecta’s Single-Cell CRISPR Screening Service\u003c\/strong\u003e (aka Perturb-Seq, CRISPR-Seq, CROP-Seq, etc.) you can study the effect of CRISPR-based perturbations on mRNA expression in many thousands of cells using single-cell RNA sequencing (scRNA-Seq) on the 10X Chromium system.  This method can provide important insights into the transcriptional response to genetic mutations and other perturbations affecting gene function.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eInvestigate transcriptional changes associated with 100+ genes in a single experiment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eCombine pooled CRISPR knockout (KO), CRISPR inhibition (CRISPRi) or CRISPR activation (CRISPRa) screening with single-cell expression analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003eIdentify genes affecting gene and pathway activation that are missed with conventional arrayed screens\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- split --\u003e\n\u003cp\u003eCellecta offers its \u003cstrong\u003eSingle-Cell CRISPR Screening Service\u003c\/strong\u003e on the 10X Chromium Controller Platform, which allows for screening of multiple genes in a single experiment and enables the identification of transcriptional profiles linked to specific gene perturbations.\u003c\/p\u003e\n\u003cp\u003eWe provide all services required to run most screens on-site, including construction and packaging of custom pooled sgRNA libraries, single-cell RNA-Seq on our in-house 10X Chromium and Illumina sequencing instruments, read alignment to identify hits, and post-screening analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSingle-Cell CRISPR Screening workflow \u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eStep 1:\u003c\/strong\u003e Clone sgRNA library\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStep 2:\u003c\/strong\u003e Cells transduced with pooled sgRNA libraries are loaded in the 10X reaction\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStep 3:\u003c\/strong\u003e Beads are co-encapsulated with unique barcodes and cells are dispersed in discrete droplets\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStep 4:\u003c\/strong\u003e The sgRNA and mRNAs labeled with bead barcodes are used to construct libraries, and running scRNA-Seq, single-cell transcriptional profiles are generated\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStep 5:\u003c\/strong\u003e Changes in gene expression associated with specific sgRNAs are linked to gene-specific knockouts\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eFor an overview of Cellecta's other functional genomics offerings, see \u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-Brochure_8.5x11-r6_WEB.pdf?v=1760647471\" title=\"Cellecta CRISPR and RNAi Functional Genomics Capabilities\" rel=\"noopener\" target=\"_blank\"\u003eCRISPR \u0026amp; RNAi Functional Genomics Capabilities\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca rel=\"noopener\" title=\"Cellecta Single-Cell CRISPR Screening Service - quote request\" href=\"https:\/\/cellecta.wufoo.com\/forms\/singlecell-crispr-screening-svcs-quote-request\/\" target=\"_blank\"\u003e\u003cbutton class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\" type=\"button\" style=\"width: 100%;\"\u003eContact Us to Discuss Your Project\u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdl class=\"faqAccordion\"\u003e\n\u003cdt\u003e\u003cbutton aria-expanded=\"false\" aria-controls=\"panel-01\" type=\"button\"\u003eMore Information\u003c\/button\u003e\u003c\/dt\u003e\n\u003cdd style=\"display: none;\" aria-hidden=\"true\" id=\"panel-01\"\u003e\n\u003cp\u003eIn addition to the full-service screening experiment, you may be interested in:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cellecta.com\/collections\/crispr-seq-barcoded-sgrna-libraries-perturb-seq-crop-seq\"\u003eCRISPR-barcoded sgRNA libraries for 27 human and mouse anti-cancer genes \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cellecta.com\/products\/custom-sgrna-libraries\"\u003eCustom sgRNA libraries \u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eData\u003c\/h3\u003e\n\u003cimg height=\"auto\" width=\"100%\" alt=\"Single-Cell CRISPR Screening Service - Quote Request\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Single-Cell_CRISPR_Screening_Service-2.jpg?v=1663939042\"\u003e\n\u003ch3\u003eOther Resources\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-Brochure_8.5x11-r6_WEB.pdf?v=1760647471\"\u003eBrochure: CRISPR \u0026amp; RNAi Functional Genomic Capabilities\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/062025_EACR-scCRISPR-A0-landscape-v4.pdf?v=1750793296\"\u003ePoster: Single-cell CRISPR screens: a comparative evaluation of droplet-based and combinatorial split-pool barcoding platforms \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/10_2022_Perturb-seq_A0.pdf?v=1665088196\"\u003ePoster: Perturb-seq analysis reveals key mediators of TNFα-induced transcriptional response \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.youtube.com\/watch?v=gXJKsi2KxEc\u0026amp;feature=youtu.be\"\u003ePresentation: Perturb-Seq analysis reveals key mediators of TNFα-induced transcriptional response (video: 20 min) by Donato Tedesco \u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Perturb-Seq-Service-Flyer-Cellecta-A4.pdf?v=1664387801\"\u003eSingle-Cell CRISPR Screening Service (Perturb-Seq, CRISP-Seq, CROP-Seq) flyer\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-Libraries-Single-Cell-Cellecta-A4.pdf?v=1664387801\"\u003eCRISPR Libraries for Single-Cell Expression Screening flyer\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cellecta.com\/blogs\/news\/rna-seq-analysis-of-pooled-crispr\"\u003eBlog Post: Cellecta Scribe™ Vectors Enable RNA-Seq Analysis of Pooled CRISPR and RNAi Screens\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cellecta.com\/blogs\/news\/perturb-seq-screening-cell-by-cell-analysis-of-gene-perturbations-induced-by-pooled-crispr-sgrna-libraries\"\u003eBlog Post: Perturb-Seq Screening: Cell-by-Cell Analysis of Gene Perturbations Induced by Pooled CRISPR sgRNA Libraries\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eRelevant Publications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eDixit A, Parnas O et al. (2016) Perturb-Seq: \u003cstrong\u003eDissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens.\u003c\/strong\u003e \u003cem\u003eCell\u003c\/em\u003e 167( 7): 1853–1866. [abstract]\u003c\/li\u003e\n\u003cli\u003eAdamson B, Norman TM et al. (2016) \u003cstrong\u003eA Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response.\u003c\/strong\u003e \u003cem\u003eCell\u003c\/em\u003e 167( 7):1867–1882. [abstract]\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/dd\u003e\n\u003c\/dl\u003e","brand":"Genetic Screens","offers":[{"title":"Default Title","offer_id":43245778534658,"sku":"CRGC-PS","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/products\/Perturb-seqworkflow-D-Cellecta_480x480_5134a85b-e7a7-4e4d-a1b0-9026216a6b94.png?v=1664383081"},{"product_id":"functional-genetic-screening-analysis","title":"Functional Genetic Screening Data Analysis","description":"\u003cp\u003e\u003ca href=\"https:\/\/cellecta.wufoo.com\/forms\/genetic-screen-analysis-quote-request\/\" title=\"Cellecta Functional Genetic Screening Bioinformatics Analysis Quote Request\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e Get A Quote \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eCellecta Inc. provides \u003cstrong\u003ecomprehensive data analysis services for functional genetic screens utilizing pooled lentiviral CRISPR and shRNA libraries sequenced at Cellecta\u003c\/strong\u003e. Get details on Cellecta's service offerings for genomic DNA isolation, sequencing, and basic guide frequency reporting, at \u003ca href=\"https:\/\/cellecta.com\/collections\/next-gen-sequencing-crispr-rnai-libraries\/products\/sequencing-service-for-samples-from-genetic-screens\" target=\"_blank\"\u003eSequencing Service for Samples from Genetic Screens\u003c\/a\u003e. \u003c\/p\u003e\n\u003cp\u003eIn addition to these core steps, Cellecta's Functional Genetic Screening Bioinformatics Analysis pipeline integrates computational tools like \u003cstrong\u003eMAGeCK, DrugZ, and custom in-house developed scripts to assess gene enrichment or depletion, identifying essential genes, gene-drug interactions, and key pathways related to specific phenotypes\u003c\/strong\u003e. The information below describes further downstream bioinformatics analysis offerings, i.e. the last step of the workflow illustrated in Fig. 1.\u003c\/p\u003e\n\u003c!-- split --\u003e\n\u003cp\u003e\u003cimg style=\"width: 100%; max-width: 600px;\" alt=\"Genetic Screen Analysis Overview\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Fxl_Screen_Analysis_Fig1.jpg?v=1741210985\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFig. 1:\u003c\/strong\u003e Overview of Genetic Screen Analysis Services\u003c\/em\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Cellecta_Functional_Screening_NGS-Bioinformatics_Analysis_Service.pdf?v=1741741566\" title=\"Cellecta Sample Functional Genetic Screening Bioinformatics Analysis Report\" rel=\"noopener\" target=\"_blank\"\u003eView Sample Functional Screening Analysis Report \u0026gt;\u003c\/a\u003e\u003c\/h3\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eBasic Report\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003eThe CRISPR Data Analysis workflow (Fig. 2) that results in our basic report includes the \u003cstrong\u003eModel-based Analysis of Genome-wide CRISPR-Cas9 Knockout (MAGeCK)\u003c\/strong\u003e essential data quality and control analyses, providing information on\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eNGS data error distribution\u003c\/li\u003e\n\u003cli\u003eNGS data nucleotide content distribution analysis\u003c\/li\u003e\n\u003cli\u003eNGS data quality control summary, and\u003c\/li\u003e\n\u003cli\u003eCRISPR screening guide quality control \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg style=\"width: 100%; max-width: 600px;\" alt=\"Basic Report Data Quality Summary\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Fxl_Screen_Analysis_Fig2.jpg?v=1741210985\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFig. 2: \u003c\/strong\u003eCRISPR Analysis Workflow \u003c\/em\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cb\u003eAdvanced Report (CRISPR Screening Analysis)\u003c\/b\u003e\u003c\/h3\u003e\n\u003cp\u003eFor more in-depth analysis, we have developed several bioinformatics modules to support the analysis of various CRISPR screening projects, an advanced report, including:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMAGeCK Analysis (Default Option)\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMAGeCK (Model-based Analysis of Genome-Wide CRISP-Cas9 Knockout) \u003c\/strong\u003eanalysis integrates both \u003cstrong\u003eRRA (Robust Rank Aggregation)\u003c\/strong\u003e and \u003cstrong\u003eMLE (Maximum Likelihood Estimation)\u003c\/strong\u003e methods to consistently identify genes with significant enrichment or depletion, offering deep insights into gene function and the pathways involved in your experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRRA and  \u003c\/strong\u003e\u003cstrong\u003eMLE\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRRA\u003c\/strong\u003e is the preferred method in MAGeCK analysis due to its ability to aggregate sgRNA ranks, and provide robust gene ranking. It effectively handles variability across guides and reduces noise, making it highly reliable for large-scale CRISPR screens. \u003cstrong\u003eMLE\u003c\/strong\u003e, on the other hand, is ideal for studies involving different isolines, offering precise, quantitative estimates of gene effects in diverse genetic backgrounds.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdditional MAGeCK features\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eOur analysis includes RRA Comparison Summaries, Volcano Plots of Selected Genes, and MLE Comparison Summaries, offering a detailed distribution of gene beta scores and identification of positively and negatively selected genes. Pathway enrichment analysis (Gene Set Enrichment Analysis (GSEA), KEGG, Gene Ontology (GO), Reactome) is also available.\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDrugZ (Drug Screening Analysis) - by request only\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eDrugZ is tailored for CRISPR screens involving drug treatments, detecting genes that influence drug sensitivity or resistance. It offers a focused approach to identifying drug-gene interactions to advance drug discovery and precision medicine.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCRISPR Tiling Analysis\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCellecta designs CRISPR tiling libraries and provides bioinformatics analysis for screens using them. This analysis technique identifies functional domains within proteins, and critical elements in annotated genes or enhancers by targeting multiple gene segments. Analysis of tiling screens helps uncover \"drop out\" regions, revealing essential genomic elements and key protein domains (Fig. 3).\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-tiling_ac578d1b-82fd-4539-be3d-b99f717b230c.jpg?v=1741744764\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eFig. 3: \u003c\/strong\u003eCRISPR tiling analysis data\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003eReady to get new insights from your functional screening data? Let us know what you need on the form to \u003ca href=\"https:\/\/cellecta.wufoo.com\/forms\/genetic-screen-analysis-quote-request\/\" title=\"Cellecta Functional Genetic Screen Bioinformatics Analysis Quote Request\"\u003erequest a quote now\u003c\/a\u003e or contact us at \u003ca href=\"mailto:info@cellecta.com\"\u003einfo@cellecta.com\u003c\/a\u003e for more information. Our expert team is happy to assist you superpower your research.\u003c\/p\u003e\n\u003cstyle\u003e\n\n  .cbb-frequently-bought-container {display: none!important;}\n  \n\u003c\/style\u003e","brand":"Bioinformatics Analysis Services","offers":[{"title":"Default Title","offer_id":45830765150466,"sku":null,"price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Pearson_correlation.jpg?v=1741918848"}],"url":"https:\/\/cellecta.com\/collections\/contract-projects.oembed","provider":"Cellecta ","version":"1.0","type":"link"}