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Catalog #: KOHGW-M123-P
Cellecta's original Genome-Wide CRISPR Knockout Pooled sgRNA Library was designed as three modules, each targeting approximately a third of the genes with eight sgRNAs per gene, which is more than most other libraries use. In addition to filtering to ensure guides are unique for each gene target to minimize false positives, the sgRNAs in Cellecta's Three-Module Human Genome-Wide CRISPR Library incorporate Cellecta's HEAT design sgRNA tracrRNA structure which has been shown to generate more robust screening results than the standard sgRNA design.
Three separate modules, each with approximately fifty thousand constructs provide a practical approach to screens with more guides per target. The modular format is similar to Cellecta’s Human Genome-Wide shRNA Library, and each of the CRISPR and RNAi Library modules target the same sets of genes so that investigators can more easily make use of both techniques for genome-wide loss of function screens.
CRISPR Human Genome Knockout Module 1 targets the most annotated genes, including members of major signal transduction pathways, genes from the Cancer Genome Atlas (TCGA), and approved drug targets.
Module 2 targets additional disease-associated genes and drug targets.
Module 3 targets cell surface markers, extracellular matrix genes, and DNA binding genes.