Catalog #: CRUTEST
The CRISPRuTest™ Functional Cas9 Activity Assay Kit can be used to measure the Cas9 nuclease activity in any mammalian cell system. The CRISPR system uses a target single-guide RNA molecule (sgRNA) to target and cut a specific sequence on the genomic DNA with the Cas9 nuclease. The CRISPRuTest Kit provides a FACS-based assay to assess the Cas9 nuclease activity in cells expressing Streptococcus pyogenes Cas9. The kit contains pseudoviral packaged lentiviral constructs with a green fluorescent protein (GFP) gene whose fluorescence is disrupted in cells with active Cas9. The kit provides enough reagents to measure this activity in 5-15 cell lines.