CRISPR and RNAi Lentiviral Library Construction

 Get the right custom library for your screen!

We offer a comprehensive range of CRISPR and RNAi custom library options that can target virtually any defined sequences and accommodate your most challenging projects

Cellecta has over 15 years of experience making high-quality sgRNA and shRNA pooled libraries. Our custom sgRNA and shRNA constructs and library service can easily accommodate standard and non-standard oligo designs for specialized applications, including

  • CRISPR KO, CRISPRa, CRISPRi, and CRISPRa SAM complex compatible libraries
  • Single-cell applications that use guides with specific capture sequences.
  • Designs that combine barcodes or unique molecular identifier (UMI) with shRNAs.
  • sgRNAs that are compatible with other CRISPR-Cas proteins.
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CRISPR and RNAi Lentiviral Library Construction

Library Construction Service
For CRISPR and RNAi Applications

Step 1. Design and Synthesis of Oligonucleotides


  • You provide your list of targets or the RefSeq identification

  • We design 3-5 sgRNAs per gene for CRISPR applications

  • We design 10 or more shRNAs to each specific transcript for RNAi applications

Design and Synthesis of Oligonucleotides

Step 2. Library Cloning Into Your Vector Of Choice


  • Vectors for constitutive or tet-inducible expression

  • Incorporate barcodes or unique molecular identifiers

  • Select from a panel of Cas9 expression constructs for CRISPR applications

  • Provide your own vectors

Library Cloning Into Your Vector Of Choice

Step 3. Deliverables: Plasmid or Packaged Libraries


  • 500 ug pooled plasmid library or VSV-g pseudotyped viral particles

  • All sequence information on guides and vectors

  • Cloning site design

  • Primer information for sequencing

  • NGS library distribution data

Deliverables: Plasmid or Packaged Libraries

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