{"product_id":"crispr-human-genome-knockout-libraries","title":"CRISPR Human Genome Knockout Libraries","description":"\u003cp\u003eCellecta’s latest \u003cstrong\u003eSingle-Module Human Genome-Wide CRISPR Knockout Libraries\u003c\/strong\u003e are designed to deliver robust knockout performance, enabling efficient pooled screening across the human genome. \u003c\/p\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli\u003eSingle-module, pooled sgRNA libraries targeting approximately 19,382 protein-coding genes and 1,859 microRNAs \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003e87K Human CRISPR Knockout Library (4 sgRNAs\/gene)\u003c\/strong\u003e comprises ~87,000 sgRNA constructs. In addition to gene-targeting guides, it includes 1,000 non-targeting controls, 400 intron-targeting controls, 400 intergenic sgRNAs, and 400 safe-harbor sgRNAs targeting 2 sites: AAVS1 and CLYBL.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e43K Human CRISPR Knockout Library (2 sgRNAs\/gene) \u003c\/strong\u003ecomprises ~43,000 sgRNA constructs. In addition, it includes 200 non-targeting controls and 200 intergenic controls, and achieves the same knockout efficiency as the 87K library despite its more compact design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli\u003eBoth the 43K and 87K libraries are designed using the criteria for effective guides described by DeWeirdt et al. 2022, which generates more robust screening results than the standard knockout 80K v2 library, assessed by the observed depletion of sgRNAs targeting common essential genes (Fig. 1)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg style=\"display: block; margin-left: auto; margin-right: auto;\" height=\"413\" width=\"455\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/43K-87K_CRISPR_KO_Libraries_vs_80Kv2.png?v=1778627819\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left; padding-left: 120px;\"\u003e\u003cb\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Calibri',sans-serif; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;\"\u003eFig 1:  \u003c\/span\u003e\u003c\/b\u003e\u003cspan style=\"font-size: 12.0pt; line-height: 107%; font-family: 'Calibri',sans-serif; mso-ascii-theme-font: minor-latin; mso-fareast-font-family: 'Times New Roman'; mso-fareast-theme-font: minor-fareast; mso-hansi-theme-font: minor-latin; mso-bidi-font-family: 'Times New Roman'; mso-bidi-theme-font: minor-bidi; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;\"\u003e\u003cstrong\u003eCellecta Next\u003c\/strong\u003e\u003cspan style=\"color: black; mso-themecolor: text1;\"\u003e\u003cstrong\u003e-Generation Human CRISPR 43K and 87K Knockout Libraries Outperform CRISPR 80Kv2 Knockout Library. \u003c\/strong\u003eThe 87K design offers 4 sgRNAs\/gene, while the 43K has a compact 2 sgRNAs\/gene design. The reduced library size enables effective screens with fewer cells, as it achieves knockout efficiencies comparable to those of the 87K Knockout library. \u003c\/span\u003e\u003cspan style=\"mso-spacerun: yes;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli\u003eGuide designs are obtained using the CRISPick web tool from the Genetic Perturbation Platform (GPP) at the Broad Institute (PMID: 36068235) and are then ranked using Cellecta's proprietary algorithm.\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThe tracrRNA portion of the sgRNA features the HECG design, an improvement over the \u003ca rel=\"noopener\" title=\"Cellecta sgRNA improved design\" href=\"\/pages\/modified-sgrna-design-improves-results-crispr-knockout-screens\" target=\"_blank\"\u003eHEAT design\u003c\/a\u003e. \u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eThese libraries may be obtained in the following vectors:\n\u003cul\u003e\n\u003cli\u003eUbiC-TagRFP and Puro (pRSG162-U6-sg-UbiC-TagRFP-2A-Puro vector)\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eUbiC-Puro (pRSGUPS2-U6-sg-UbiC-Puro vector)\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli style=\"list-style-type: none;\"\u003e\n\u003cul\u003e\n\u003cli\u003ePlasmid and lentiviral packaged formats \n\u003cul\u003e\n\u003cli\u003e200 ug plasmid\u003c\/li\u003e\n\u003cli\u003e2 x 10^8 TU or 1 x 10^9 TU\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003e*NOTE:\u003c\/strong\u003e Researchers who would like to purchase more units of our older libraries may contact us at \u003ca href=\"mailto:info@cellecta.com\" rel=\"noopener\" target=\"_blank\"\u003einfo@cellecta.com\u003c\/a\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003ca name=\"tabs\" id=\"tabs\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdl class=\"faqAccordion\"\u003e\n\u003cdt\u003e\u003cbutton type=\"button\" aria-controls=\"panel-01\" aria-expanded=\"false\"\u003eManual and Product Documentation\u003c\/button\u003e\u003c\/dt\u003e\n\u003cdd id=\"panel-01\" aria-hidden=\"true\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBrochure:\u003c\/strong\u003e \u003ca rel=\"noopener\" title=\"Cellecta CRISPR and RNAi Functional Genomics Capabilities\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/CRISPR-Brochure_8.5x11-r6_WEB.pdf?v=1760647471\" target=\"_blank\"\u003eCellecta CRISPR \u0026amp; RNAi Functional Genomics Capabilities\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene List: \u003c\/strong\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Cellecta-CRISPR-KOHGW-87K-sgRNA-Library-Gene-List.xlsx?v=1778628536\" title=\"Cellecta CRISPR Human Genome 87K Knockout Library Gene List\" rel=\"noopener\" target=\"_blank\"\u003eCRISPR Human Genome 87K Knockout Library Gene List\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Cellecta-CRISPR-KOHGW-43K-sgRNA-Library-Gene-List.xlsx?v=1778628524\" title=\"Cellecta Human Genome-Wide 43K CRISPR Knockout Library Gene List\" target=\"_blank\"\u003eCRISPR Human Genome 43K Knockout Library Gene List\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eManual: \u003c\/strong\u003e\u003ca href=\"https:\/\/manuals.cellecta.com\/crispr-pooled-lentiviral-sgrna-libraries\/\" title=\"Cellecta CRISPR Pooled Lentiviral sgRNA Libraries Manual\"\u003eCRISPR Pooled Lentiviral sgRNA Libraries Manual\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/dd\u003e\n\u003cdt\u003e\u003cbutton type=\"button\" aria-controls=\"panel-02\" aria-expanded=\"false\"\u003eLibrary Vector Information\u003c\/button\u003e\u003c\/dt\u003e\n\u003cdd id=\"panel-02\" aria-hidden=\"false\"\u003e\n\u003cdiv style=\"clear: both;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"TablePress\" data-table=\"61504\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cem\u003e* Sequences are available in FASTA (.txt), GenBank (.gb), and SnapGene (.dna) formats. You may use the\u003cspan\u003e \u003c\/span\u003e\u003ca title=\"Link to snapgene.com\" href=\"https:\/\/www.snapgene.com\/products\/snapgene_viewer\/\"\u003efree SnapGene Viewer\u003c\/a\u003e\u003cspan\u003e \u003c\/span\u003eto open .dna files. If you would like vector files in another format, please\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"mailto:info@cellecta.com\"\u003econtact us\u003c\/a\u003e. To download .txt and .gb files, right click on the link and Save As. \u003c\/em\u003e\u003c\/div\u003e\n\u003c\/dd\u003e\n\u003cdt\u003e\u003cbutton type=\"button\" aria-controls=\"panel-03\" aria-expanded=\"false\"\u003eSearch Product Certificates by Lot# or Catalog#\u003c\/button\u003e\u003c\/dt\u003e\n\u003cdd id=\"panel-03\" aria-hidden=\"false\"\u003e\n\u003cdiv style=\"clear: both;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"TablePress\" data-table=\"5995\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/dd\u003e\n\u003cdt\u003e\u003cbutton type=\"button\" aria-controls=\"panel-04\" aria-expanded=\"false\"\u003eAlignment Software Supporting Files\u003c\/button\u003e\u003c\/dt\u003e\n\u003cdd id=\"panel-04\" aria-hidden=\"true\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eLibrary Configuration Files (JSON format, compressed): \u003c\/strong\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Cellecta Human 87K CRISPR Knockout Library JSON files\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Cellecta-CONFIG-KOHGW-87K-sgRNA-Library-LNGS-120.zip?v=1778629126\" target=\"_blank\"\u003eKOHGW-87k Libraries\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca rel=\"noopener\" title=\"Cellecta Human 43K CRISPR Knockout Library JSON files\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/Cellecta-CONFIG-KOHGW-43K-sgRNA-Library-LNGS-120.zip?v=1778629482\" target=\"_blank\"\u003eKOHGW-43k Libraries\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Description Input Form: \u003c\/strong\u003e\u003ca href=\"https:\/\/cdn.cellecta.com\/software\/NGS-Sample-Description-Input-Form.xlsm\" target=\"_blank\"\u003eXLSM (Please enable macros)\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/dd\u003e\n\u003c\/dl\u003e","brand":"Library","offers":[{"title":"87K Human CRISPR Knockout \/ UbiC-RFP-Puro selection \/ 200ug Plasmid","offer_id":47759345221890,"sku":"KOHGW-87K-P","price":9500.0,"currency_code":"USD","in_stock":true},{"title":"87K Human CRISPR Knockout \/ UbiC-Puro selection \/ 200ug Plasmid","offer_id":47759345287426,"sku":"KOHGW-UP-87K-P","price":9500.0,"currency_code":"USD","in_stock":true},{"title":"43K Human CRISPR Knockout \/ UbiC-RFP-Puro selection \/ 200ug Plasmid","offer_id":47763331383554,"sku":"KOHGW-43K-P","price":8500.0,"currency_code":"USD","in_stock":true},{"title":"43K Human CRISPR Knockout \/ UbiC-RFP-Puro selection \/ 2x10^8TU Virus","offer_id":47763331416322,"sku":"KOHGW-43K-V8","price":8500.0,"currency_code":"USD","in_stock":true},{"title":"43K Human CRISPR Knockout \/ UbiC-RFP-Puro selection \/ 10^9TU Virus","offer_id":47763331449090,"sku":"KOHGW-43K-V9","price":18500.0,"currency_code":"USD","in_stock":true},{"title":"43K Human CRISPR Knockout \/ UbiC-Puro selection \/ 200ug Plasmid","offer_id":47763331481858,"sku":"KOHGW-UP-43K-P","price":8500.0,"currency_code":"USD","in_stock":true},{"title":"43K Human CRISPR Knockout \/ UbiC-Puro selection \/ 2x10^8TU Virus","offer_id":47763331514626,"sku":"KOHGW-UP-43K-V8","price":8500.0,"currency_code":"USD","in_stock":true},{"title":"43K Human CRISPR Knockout \/ UbiC-Puro selection \/ 10^9TU Virus","offer_id":47763331547394,"sku":"KOHGW-UP-43K-V9","price":18500.0,"currency_code":"USD","in_stock":true},{"title":"87K Human CRISPR Knockout \/ UbiC-RFP-Puro selection \/ 10^9TU Virus","offer_id":47763435061506,"sku":"KOHGW-87K-V9","price":19500.0,"currency_code":"USD","in_stock":true},{"title":"87K Human CRISPR Knockout \/ UbiC-Puro selection \/ 2x10^8TU Virus","offer_id":47763449774338,"sku":"KOHGW-UP-87K-V8","price":9500.0,"currency_code":"USD","in_stock":true},{"title":"87K Human CRISPR Knockout \/ UbiC-RFP-Puro selection \/ 2x10^8TU Virus","offer_id":47763458851074,"sku":"KOHGW-87K-V8","price":9500.0,"currency_code":"USD","in_stock":true},{"title":"87K Human CRISPR Knockout \/ UbiC-Puro selection \/ 10^9TU Virus","offer_id":47763462455554,"sku":"KOHGW-UP-87K-V9","price":19500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/KOHGW-UP-87K-V8_afba4226-ebce-47b5-b9f1-54aa67733ed1.png?v=1778624884","url":"https:\/\/cellecta.com\/products\/crispr-human-genome-knockout-libraries","provider":"Cellecta ","version":"1.0","type":"link"}