{"product_id":"drivermap-cell-free-air-rna-dna-profiling-service","title":"DriverMap™ Cell-Free Adaptive Immune Receptor (cfAIR) RNA \u0026 DNA Repertoire Profiling Service","description":"\u003cp\u003e\u003ca href=\"https:\/\/cellecta.wufoo.com\/forms\/drivermap-cellfree-air-profiling-quote-request\/\" title=\"Cellecta Cell-Free Adaptive Immune Receptor (cfAIR) TCR-BCR Repertoire Profiling Service\" rel=\"noopener\" target=\"_blank\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e Get A Quote \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eThe DriverMap™ Cell-free Adaptive Immune Receptor (cfAIR) Repertoire Profiling Service profiles \u003cstrong\u003eall TCR and BCR CDR3 regions\u003c\/strong\u003e from \u003cstrong\u003ecell-free RNA and DNA in human plasma\u003c\/strong\u003e. Send us frozen plasma, and we purify the cell-free nucleic acids, perform library prep, sequence the libraries, and deliver a comprehensive analysis report — no biopsy, no dealing with live cells, and no in-house NGS workflow required.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eLiquid-biopsy TCR\/BCR profiling from as little as \u003cstrong\u003e1 mL of plasma\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTwo data sets from one sample:\u003c\/strong\u003e cfRNA (lymphocyte activation) and cfDNA (lymphocyte turnover), separated by barcodes from a single sample, in the same workflow\u003c\/li\u003e\n\u003cli\u003eCaptures clonotypes from \u003cstrong\u003etumor-infiltrating and tissue-resident lymphocytes\u003c\/strong\u003e that blood-cell adaptive immune receptor (AIR) repertoire profiling does not show\u003c\/li\u003e\n\u003cli\u003eComplete end-to-end service from plasma to report, with a turnaround of as little as 3 weeks\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- split --\u003e\n\u003cp\u003eThe \u003cstrong\u003eDriverMap™ Cell-Free AIR service workflow \u003c\/strong\u003eis designed to obtain the clonotype repertoire:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eCo-purify cfRNA from extracellular vesicles, exosomes, and the soluble plasma fraction together with nucleosome-protected cfDNA, using the \u003ca href=\"\/products\/drivermap-cell-free-rna-dna-purification-kit\" title=\"Cellecta DriverMap Cell-Free RNA and DNA Purification Kit\" rel=\"noopener\" target=\"_blank\"\u003eDriverMap™ Cell-Free RNA \u0026amp; DNA Purification Kit.\u003c\/a\u003e\n\u003c\/li\u003e\n\u003cli\u003eAmplify the CDR3 region of \u003cstrong\u003eall seven TCR and BCR chains\u003c\/strong\u003e (TRA, TRB, TRG, TRD, IGH, IGK, IGL) with short FR3–J amplicons of approximately 170 bp designed for fragmented cell-free templates.\u003c\/li\u003e\n\u003cli\u003eCount template molecules and correct PCR and sequencing errors with \u003cstrong\u003eValidator Barcodes (VBCs)\u003c\/strong\u003e, for accurate detection of rare clonotypes present at as few as 1–10 copies.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cellecta.wufoo.com\/forms\/drivermap-cellfree-air-profiling-quote-request\/\" title=\"DriverMap Cell Free Adaptive Immune Receptor (cfAIR) TCR BCR Repertoire Profiling Service\" rel=\"noopener\" target=\"_blank\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/AIR_Plasma_Service_Workflow_2.png\" alt=\"DriverMap Cell-Free AIR Profiling Service workflow: send plasma, Cellecta runs the complete workflow, receive data and a report\" width=\"100%\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003eSample Requirements\u003c\/h3\u003e\n\u003ctable width=\"100%\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSample\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eRequirement\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePlasma (required)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e1–6 mL per sample; 4–5 mL recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eBlood collection\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eK2 EDTA tubes (ACD-A or PPT tubes also accepted). Do not use blood-stabilizing tubes (for example, Streck Nucleic Acid BCT or PAXgene Blood ccfDNA).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003ePlasma preparation\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eProcess within 2–4 hours of collection. Double spin: 2,500 × g, 15 min, 20 °C, no brake (twice). Freeze aliquots at –80 °C. Avoid freeze–thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMatched control (recommended)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBuffy coat or PBMC (or 100 ng purified RNA); FFPE tumor tissue (or 500–1,000 ng purified RNA)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eShipping\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFrozen on dry ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch3\u003eSome Applications\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cstrong\u003eCancer Immunotherapy Monitoring\u003c\/strong\u003e\u003cbr\u003eTrack tumor-reactive TCR and BCR clonotypes in plasma before and during checkpoint-inhibitor therapy, without the need for invasive biopsy.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLymphoid Malignancy Detection\u003c\/strong\u003e\u003cbr\u003eDetect and follow malignant B-cell and T-cell clones in plasma cfDNA and cfRNA.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiobanked Plasma Characterization\u003c\/strong\u003e\u003cbr\u003eAdd immune repertoire data to further characterize plasma already collected for ctDNA or other liquid-biopsy studies.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eFor laboratories looking to run the assays in-house, learn about the \u003ca href=\"\/products\/drivermap-cell-free-air-rna-dna-tcr-bcr-profiling-kit\" target=\"_blank\" title=\"DriverMap Cell-Free Adaptive Immune Receptor (cfAIR) TCR-BCR Repertoire Profiling Assay\" rel=\"noopener\"\u003e\u003cstrong\u003eDriverMap cfAIR TCR-BCR Repertoire Profiling Kit here.\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003eResources\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003ePoster:\u003c\/strong\u003e \u003cspan\u003e\u003cem\u003ePlasma cell-free nucleic acid profiling of TCR and BCR repertoires reveal distinct contributions of cfDNA and cfRNA to adaptive immune monitoring in cancer\u003c\/em\u003e, October 2026 (\u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/2610_cfDNA-11x17-Portrait-v3.pdf?v=1792449626\" rel=\"noopener\" target=\"_blank\"\u003ePDF\u003c\/a\u003e\u003cspan\u003e)\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cellecta.wufoo.com\/forms\/drivermap-cellfree-air-profiling-quote-request\/\" title=\"Cellecta Cell-Free Adaptive Immune Receptor (cfAIR) TCR-BCR Repertoire Profiling Service\" rel=\"noopener\" target=\"_blank\"\u003e\u003cbutton style=\"width: 100%;\" type=\"button\" class=\"shopify-payment-button__button shopify-payment-button__button--unbranded\"\u003e Get A Quote \u003c\/button\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"TCR-BCR Repertoire Profiling","offers":[{"title":"Default Title","offer_id":50751902646530,"sku":null,"price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/AIR_Plasma_Service_Workflow_2.png?v=1791995327","url":"https:\/\/cellecta.com\/products\/drivermap-cell-free-air-rna-dna-profiling-service","provider":"Cellecta ","version":"1.0","type":"link"}