{"product_id":"drivermap-cell-free-air-rna-dna-tcr-bcr-profiling-kit","title":"DriverMap™ Cell-Free AIR RNA \u0026 DNA TCR-BCR Profiling Kit","description":"\u003cp\u003eCellecta's \u003cstrong\u003eDriverMap™ Cell-Free Adaptive Immune Receptor (cfAIR) RNA \u0026amp; DNA TCR–BCR Profiling Kit\u003c\/strong\u003e (Human) is an easy-to-run, \u003cstrong\u003esingle-day,\u003c\/strong\u003e multiplex PCR-NGS assay that profiles the \u003cstrong\u003eT-cell and B-cell receptor repertoire from human plasma\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eCompared with the bulk DriverMap™ AIR RNA and DNA kits, which start from blood cells or tissue, the cell-free AIR assay starts with cell-free nucleic acid samples from plasma to profile fragmented cell-free RNA (cfRNA) and cell-free DNA (cfDNA) from a shorter ~170 bp CDR3 region of all seven TCR and BCR chains (TRA, TRB, TRG, TRD, IGH, IGK, IGL) from at least 1 ml of plasma.\u003c\/p\u003e\n\u003ch3\u003eKey Features\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eCombined cfDNA + cfRNA immune receptor profiling:\u003c\/strong\u003e Two barcoded hybridization reactions give two signals from the same plasma sample. cfDNA-based TCR\/BCR sequences capture lymphocyte turnover (apoptosis and necrosis), while cfRNA TCR\/BCR sequences measure transcripts from extracellular vesicles (EVs) which reflect lymphocyte activation. Key biomarkers are obtained without invasive biopsies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eQuantitative detection:\u003c\/strong\u003e Amplicons for each primer are tagged with Validator Barcodes (VBCs), which allows one to count template molecules and correct errors in the data deconvolution step. Fig 1 shows the number of TCR\/BCR clonotypes detected in the plasma sample from healthy PBMC Control RNA and three types of cancer patients.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOne Kit, Multiple Sample Types:\u003c\/strong\u003e Matched control RNA from buffy coat\/PBMC or FFPE tissue may also be profiled using the same protocol to identify tumor-derived and bystander clonotypes in the cfRNA or cfDNA repertoire.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eEach kit includes TCR and BCR primer sets, enzymes, buffers, a Positive Control RNA and custom NGS sequencing primers. Universal Unique Dual-Index (UDI) plates, which are required for the 2\u003csup\u003end\u003c\/sup\u003e PCR Step, need to be purchased separately \u003ca href=\"\/products\/universal-unique-dual-index-udi-ngs-plates?variant=47608896225538\"\u003ehere\u003c\/a\u003e.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eThis workflow requires 3 separate products\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cstrong\u003e\u003ca href=\"\/products\/drivermap-cell-free-rna-dna-purification-kit\" title=\"Cellecta DriverMap Cell Free RNA DNA Purification Kit\" rel=\"noopener\" target=\"_blank\"\u003eDriverMap™ Cell-Free RNA \u0026amp; DNA Purification Kit\u003c\/a\u003e \u003c\/strong\u003e(Recommended; although other commercially available purification kits may be used)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDriverMap™ Cell-Free AIR RNA \u0026amp; DNA TCR-BCR Profiling Kit \u003c\/strong\u003e(Required)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eUniversal Unique Dual Index (UDI) NGS Plate (Set A, B, C or D) \u003c\/strong\u003e(Required)\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eEach product listed above is sold separately. The tables below list all three. You can purchase all three bundled at a 20% discount. Please contact quotes@cellecta.com to request this discount.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDriverMap™ Cell-Free AIR Profiling Kits\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%; text-align: center;\"\u003e\u003cstrong\u003eCatalog #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 42%; text-align: center;\"\u003e\u003cstrong\u003eDriverMap™ Cell-Free AIR Profiling Kit\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21%; text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%;\"\u003eDMAIR2-CFTB-24-B1\u003c\/td\u003e\n\u003ctd style=\"width: 42%;\"\u003eDriverMap™ Cell-Free AIR RNA \u0026amp; DNA TCR-BCR Profiling Kit (Human), 24\u003c\/td\u003e\n\u003ctd style=\"width: 21%;\"\u003e1 kit (for 24 samples)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003ca href=\"\/products\/drivermap-cell-free-air-rna-dna-tcr-bcr-profiling-kit?variant=50751901761794\" rel=\"noopener\" title=\"Cellecta DriverMap cfAIR TCR-BCR Repertoire Profiling Assay (Human) - 24 samples\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%;\"\u003eDMAIR2-CFTB-96-B1\u003c\/td\u003e\n\u003ctd style=\"width: 42%;\"\u003eDriverMap™ Cell-Free AIR RNA \u0026amp; DNA TCR-BCR Profiling Kit (Human), 96\u003c\/td\u003e\n\u003ctd style=\"width: 21%;\"\u003e1 kit (for 96 samples)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003ca href=\"\/products\/drivermap-cell-free-air-rna-dna-tcr-bcr-profiling-kit?variant=50751901794562\" rel=\"noopener\" title=\"Cellecta DriverMap cfAIR TCR-BCR Repertoire Profiling Assay (Human) - 96 samples\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRequired: DriverMap™ Universal UDI NGS Plates (one plate per 96 samples)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%; text-align: center;\"\u003e\u003cstrong\u003eCatalog #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 42%; text-align: center;\"\u003e\u003cstrong\u003eUniversal Unique Dual Index (UDI) NGS Plate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 21%; text-align: center;\"\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003cbr\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%;\"\u003eDM-NGS-SetA-B2\u003c\/td\u003e\n\u003ctd style=\"width: 42%;\"\u003eUnique Dual Index Set A for DriverMap EXP or AIR (1-96)\u003c\/td\u003e\n\u003ctd style=\"width: 21%;\"\u003e1x 96-Well Plate (4 rxn\/well)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003ca href=\"\/products\/universal-unique-dual-index-udi-ngs-plates?variant=47608896225538\" rel=\"noopener\" title=\"Cellecta Universal UDI Plates for DriverMap Assay - Set A\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%;\"\u003eDM-NGS-SetB-B2\u003c\/td\u003e\n\u003ctd style=\"width: 42%;\"\u003eUnique Dual Index Set B for DriverMap EXP or AIR (97-192)\u003c\/td\u003e\n\u003ctd style=\"width: 21%;\"\u003e1x 96-Well Plate (4 rxn\/well)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003ca href=\"\/products\/universal-unique-dual-index-udi-ngs-plates?variant=47608917033218\" rel=\"noopener\" title=\"Cellecta Universal UDI Plates for DriverMap Assay - Set B\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%;\"\u003eDM-NGS-SetC-B2\u003c\/td\u003e\n\u003ctd style=\"width: 42%;\"\u003eUnique Dual Index Set C for DriverMap EXP or AIR (193-288)\u003c\/td\u003e\n\u003ctd style=\"width: 21%;\"\u003e1x 96-Well Plate (4 rxn\/well)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003ca href=\"\/products\/universal-unique-dual-index-udi-ngs-plates?variant=47608917065986\" rel=\"noopener\" title=\"Cellecta Universal UDI Plates for DriverMap Assay - Set C\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 18.8%;\"\u003eDM-NGS-SetD-B2\u003c\/td\u003e\n\u003ctd style=\"width: 42%;\"\u003eUnique Dual Index Set D for DriverMap EXP or AIR (289-384)\u003c\/td\u003e\n\u003ctd style=\"width: 21%;\"\u003e1x 96-Well Plate (4 rxn\/well)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2%;\"\u003e\u003ca href=\"\/products\/universal-unique-dual-index-udi-ngs-plates?variant=47608917098754\" rel=\"noopener\" title=\"Cellecta Universal UDI Plates for DriverMap Assay - Set D\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRecommended: DriverMap™ Cell-Free RNA \u0026amp; DNA Purification Kit\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor optimal yield of cfRNA and cfDNA for downstream immune profiling, we recommend using the Cellecta \u003ca href=\"\/products\/drivermap-cell-free-rna-dna-purification-kit\"\u003eCell-Free RNA \u0026amp; DNA Purification Kit\u003c\/a\u003e, which has been specifically designed for plasma purification and immune profiling workflows requiring shorter amplicons. Our experience has shown that this kit is more effective than others on the market; however, alternative off-the-shelf purification kits may also be used for this required purification step.\u003c\/p\u003e\n\u003ctable width=\"100%\" style=\"width: 99.9929%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.2096%;\"\u003e\u003cstrong\u003eCatalog #\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 32.7592%;\"\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 26.4706%;\"\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 9.21353%;\"\u003e\u003cstrong\u003eAmount\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.32784%;\"\u003e\u003cstrong\u003ePrice\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 7.88179%;\"\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 15.2096%;\"\u003eDMAIR2-CFP-24-B1\u003c\/td\u003e\n\u003ctd style=\"width: 32.7592%;\"\u003eDriverMap™ Cell-Free RNA \u0026amp; DNA Purification Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.4706%;\"\u003eCo-purifies cfRNA + cfDNA from human plasma\u003c\/td\u003e\n\u003ctd style=\"width: 9.21353%;\"\u003e25 preps\u003c\/td\u003e\n\u003ctd style=\"width: 7.32784%;\"\u003e$1,200\u003c\/td\u003e\n\u003ctd style=\"width: 7.88179%;\"\u003e\u003ca href=\"\/products\/drivermap-cell-free-rna-dna-purification-kit\" title=\"Cellecta DriverMap Cell Free RNA and DNA Purification Kit\" rel=\"noopener\" target=\"_blank\"\u003eOrder Now \u0026gt;\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/cfAIR_Kit_Fig1_Clonotypes.png\" alt=\"DriverMap cfAIR RNA and DNA TCR-BCR Profiling Kit clonotypes detected from plasma\" width=\"100%\" style=\"max-width: 800px;\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFig. 1. DriverMap cfAIR RNA \u0026amp; DNA TCR-BCR Profiling Kit shows\u003c\/strong\u003e \u003cstrong\u003eCDR3 clonotypes\u003c\/strong\u003e detected from 1 mL of plasma from 3 healthy donors and 6 cancer patients (NSCLC, melanoma, HCC) compared with matched FFPE tumor RNA (500 ng) and healthy PBMC control RNA (50 ng). Cell-free nucleic acid (cfNA), which combines cfDNA \u0026amp; cfRNA yields 10-fold more clonotypes than cfDNA alone. Matched FFPE tumor RNA yields a higher number of clonotypes due to tumor lymphocyte content, with overlapping clonotypes in FFPE and cfNA indicating apoptosis and immune activation.\u003c\/p\u003e\n\u003ch3\u003eFull-Service Option\u003c\/h3\u003e\n\u003cp\u003eThe \u003cstrong\u003e\u003ca href=\"\/products\/drivermap-cell-free-air-rna-dna-profiling-service\" title=\"Cellecta DriverMap Cell-Free Adaptive Immune Receptor (cfAIR) TCR and BCR Repertoire Profiling Service\" rel=\"noopener\" target=\"_blank\"\u003eDriverMap Cell-Free AIR (cfAIR) TCR-BCR Profiling Service\u003c\/a\u003e\u003c\/strong\u003e is an end-to-end, sample-to-comprehensive data option available for those who would prefer to send their samples to Cellecta for repertoire profiling.\u003c\/p\u003e\n\u003ch3\u003eResources\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eUser Manual:\u003c\/strong\u003e \u003cstrong\u003e\u003ca href=\"https:\/\/manuals.cellecta.com\/drivermap-air-cell-free-ffpe-dna-rna-tcr-bcr-profiling-kit-human-v2-1\/\" title=\"Cellecta DriverMap Cell-Free Adaptive Immune Receptor (cfAIR) TCR BCR RNA \u0026amp; DNA Profiling Kit User Manual\" rel=\"noopener\" target=\"_blank\"\u003eDriverMap™ AIR Cell-Free TCR\/BCR RNA \u0026amp; DNA Profiling Kit User Manual\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePoster:\u003c\/strong\u003e  \u003cspan\u003e\u003cem\u003ePlasma cell-free nucleic acid profiling of TCR and BCR repertoires reveal distinct contributions of cfDNA and cfRNA to adaptive immune monitoring in cancer\u003c\/em\u003e, October 2026 (\u003c\/span\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/2610_cfDNA-11x17-Portrait-v3.pdf?v=1792449626\" rel=\"noopener\" target=\"_blank\"\u003ePDF\u003c\/a\u003e\u003cspan\u003e)\u003c\/span\u003e \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTechnology Guide\u003c\/strong\u003e: \u003ca href=\"https:\/\/manuals.cellecta.com\/drivermap-cell-free-rna-and-dna-immune-repertoire-profiling-technology-guide\/\" target=\"_blank\" title=\"DriverMap Cell-free RNA \u0026amp; DNA Immune Repertoire Profiling Technology Guide\" rel=\"noopener\"\u003e\u003cstrong\u003eDriverMap™ Cell-free RNA \u0026amp; DNA Immune Repertoire Profiling Technology Guide\u003c\/strong\u003e\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Kit","offers":[{"title":"24 samples \/ -20C | 1 day","offer_id":50751901761794,"sku":"DMAIR2-CFTB-24-B1","price":1800.0,"currency_code":"USD","in_stock":true},{"title":"96 samples \/ -20C | 1 day","offer_id":50751901794562,"sku":"DMAIR2-CFTB-96-B1","price":5850.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0011\/6650\/8122\/files\/DMAIR2-CFTB-24-B1.png?v=1792687088","url":"https:\/\/cellecta.com\/products\/drivermap-cell-free-air-rna-dna-tcr-bcr-profiling-kit","provider":"Cellecta ","version":"1.0","type":"link"}