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Cellecta’s LINE-1 Assay Kit provides a convenient, efficient, and targeted Next-Generation Sequencing (NGS) based approach to identify polymorphic insertion sites of LINE-1 in the human genome. The LINE-1 Assay Kit includes all the components needed to detect and identify novel LINE-1-induced genomic variations in genomic DNA isolated from tumors, blood, cells or other samples. Only a few hundred nanograms of DNA are required.
Amplify and prep of all LINE-1 elements in a sample for profiling with a single-reaction protocol
Measure the activation of retrotransposon activity in biological samples and tumor extracts simply and easily
Analyze LINE-1 insertions to discover novel oncogenic drivers and study the evolution of tumor cell populations
Reference: Streva, V. A., Jordan, V. E., Linker, S., Hedges, D. J., Batzer, M. A., & Deininger, P. L. (2015). Sequencing, identification and mapping of primed L1 elements (SIMPLE) reveals significant variation in full length L1 elements between individuals. BMC Genomics, 16(1). http://doi.org/10.1186/s12864-015-1374-y
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