Catalog #: LNGS-905

NGS Prep Kit for sgRNA Libraries in LentiCRISPRv2 (GeCKO, Brunello Kinome)

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Product Information

Cellecta’s NGS Prep Kit for sgRNA Libraries cloned into the LentiCRISPRv2 vector (e.g., single-vector CRISPR GeCKO, Brunello) provides a protocol and reagents for the PCR amplification from genomic DNA and NGS sequencing of sgRNA sequence cassette from biological samples screened using these libraries. Sufficient reagents are provided to process 12-48 DNA samples (depending on the amount of DNA/sample) with unique indexing primers to multiplex up to 12 samples run in a single lane. Software provided with the Kit enable demultiplexing of the FASTQ files.

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