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    Cellecta
    • Products
      • CRISPR & RNAi Libraries
        • CRISPR Knockout Pooled Lentiviral sgRNA Libraries
        • CRISPRa / CRISPRi Pooled Lentiviral sgRNA Libraries
        • Custom sgRNA Libraries
        • shRNA Knockdown Pooled Lentiviral Libraries
        • Custom shRNA Libraries
        • Single-Cell Perturb-Seq Libraries
      • CRISPR/RNAi Support Products
        • Cas9 Cell Lines: Pre-made & Validated
        • Custom Cas9 Cell Line Service
        • CRISPR-Test Cas9 Activity Assays
        • CRISPR sgRNA Cloning Vectors
        • CRISPR sgRNA Control Constructs
        • NGS Prep Kits for sgRNA / shRNA Libraries
        • NGS Analysis Services for Libraries
        • Bioinformatics Analysis of Screened Samples
        • RNAi shRNA Control Constructs
        • Cas9 and dCas9 Expression constructs
        • LentiTrans Transduction Reagent
      • CRISPR/RNAi Screening Services
        • CRISPR / RNAi Screening Services
        • Single-Cell CRISPR Screening Services
        • Bioinformatics Analysis of Screened Samples
      • Cell Lines
        • Pre-made Cas9 Cell Lines
        • Cas9 Cell Line Service
        • Knock-in / Knockout Cell Line Service
        • Virus Receptor Cell Line Service
        • Custom ORF Expression Cells
      • CloneTracker™ Cell Barcoding Libraries & Constructs
        • CloneTracker™ Cell Barcoding Libraries
        • Single Barcode Contructs to Label Cell Populations
        • Custom Barcode Libraries
        • NGS Prep Kits for Barcoded Libraries
        • NGS Analysis Services for Libraries
      • Lentiviral Constructs
        • Cas9 and dCas9-Variant Constructs
        • CRISPR sgRNA Control Constructs
        • RNAi shRNA Control Constructs
        • Promoter Reporter Constructs: PromoterTest Panel
        • Custom cDNA Expression Constructs
        • Custom sgRNA Constructs
        • Custom shRNA Constructs
      • Lentiviral Packaging
        • Lentiviral Vector Packaging Mix
        • LentiFuge™ Viral Concentration Reagent
        • LentiTrans™ Transduction Reagent (Polybrene for Transduction)
        • LentiPrep™ Lentiviral Reagents with Fluorescent Control for Packaging & Transduction
        • Lentiviral Packaging Services
      • Cloning Vectors
        • CRISPR sgRNA Vectors
        • RNAi shRNA Vectors
        • Tet-Inducible Systems and Vectors
      • DriverMap™ AIR TCR / BCR Repertoire Profiling
        • AIR TCR / BCR Profiling Kits
        • AIR Profiling RNA Spike-In Controls
        • Single-Cell AIR T-Cell and B-Cell Receptor Profiling Kits
        • Bioinformatics Analysis of DriverMap AIR Assay
      • DriverMap™ EXP Targeted RNA-Seq Expression Profiling
        • Human Genome-Wide EXP Expression Profiling Kit
        • Mouse Targeted EXP Expression Profiling Kit
        • Bioinformatics Analysis of DriverMap EXP Assay
    • Services
      • Cell Engineering
      • Custom Libraries
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      • Lentiviral Constructs
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      • Tissue 2 Target Service
      • Bioinformatics Analysis Services
      • DriverMap AIR / EXP Analysis Services
        • AIR Adaptive Immune Receptor TCR / BCR Profiling Service
        • Single-Cell AIR TCR / BCR Profiling Service
        • Targeted RNA-Seq Expression Profiling (EXP) Service
        • Custom Development of DriverMap™ EXP Assay
        • Bioinformatics Analysis of DriverMap EXP or RNA-Seq data
        • Bioinformatics Analysis of Bulk or Single-Cell AIR Profiling Assay
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    • CRISPR & RNAi Libraries
    • CRISPR Knockout Pooled Lentiviral sgRNA Libraries
    • CRISPRa / CRISPRi Pooled Lentiviral sgRNA Libraries
    • Custom sgRNA Libraries
    • shRNA Knockdown Pooled Lentiviral Libraries
    • Custom shRNA Libraries
    • Single-Cell Perturb-Seq Libraries
    • CRISPR/RNAi Support Products
    • Cas9 Cell Lines: Pre-made & Validated
    • Custom Cas9 Cell Line Service
    • CRISPR-Test Cas9 Activity Assays
    • CRISPR sgRNA Cloning Vectors
    • CRISPR sgRNA Control Constructs
    • NGS Prep Kits for sgRNA / shRNA Libraries
    • NGS Analysis Services for Libraries
    • Bioinformatics Analysis of Screened Samples
    • RNAi shRNA Control Constructs
    • Cas9 and dCas9 Expression constructs
    • LentiTrans Transduction Reagent
    • CRISPR/RNAi Screening Services
    • CRISPR / RNAi Screening Services
    • Single-Cell CRISPR Screening Services
    • Bioinformatics Analysis of Screened Samples
    • Cell Lines
    • Pre-made Cas9 Cell Lines
    • Cas9 Cell Line Service
    • Knock-in / Knockout Cell Line Service
    • Virus Receptor Cell Line Service
    • Custom ORF Expression Cells
    • CloneTracker™ Cell Barcoding Libraries & Constructs
    • CloneTracker™ Cell Barcoding Libraries
    • Single Barcode Contructs to Label Cell Populations
    • Custom Barcode Libraries
    • NGS Prep Kits for Barcoded Libraries
    • NGS Analysis Services for Libraries
    • Lentiviral Constructs
    • Cas9 and dCas9-Variant Constructs
    • CRISPR sgRNA Control Constructs
    • RNAi shRNA Control Constructs
    • Promoter Reporter Constructs: PromoterTest Panel
    • Custom cDNA Expression Constructs
    • Custom sgRNA Constructs
    • Custom shRNA Constructs
    • Lentiviral Packaging
    • Lentiviral Vector Packaging Mix
    • LentiFuge™ Viral Concentration Reagent
    • LentiTrans™ Transduction Reagent (Polybrene for Transduction)
    • LentiPrep™ Lentiviral Reagents with Fluorescent Control for Packaging & Transduction
    • Lentiviral Packaging Services
    • Cloning Vectors
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    • AIR Profiling RNA Spike-In Controls
    • Single-Cell AIR T-Cell and B-Cell Receptor Profiling Kits
    • Bioinformatics Analysis of DriverMap AIR Assay
    • DriverMap™ EXP Targeted RNA-Seq Expression Profiling
    • Human Genome-Wide EXP Expression Profiling Kit
    • Mouse Targeted EXP Expression Profiling Kit
    • Bioinformatics Analysis of DriverMap EXP Assay
    • Cell Engineering
    • Custom Libraries
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    • Targeted RNA-Seq Expression Profiling (EXP) Service
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    Custom Pooled Lentiviral Libraries

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    Oligonucleotide and gene synthesis technology have largely made obsolete the more traditional techniques to clone genomic DNA and RNA isolated directly from organisms. Traditional cDNA, genomic, and other fragment libraries have become antiquated, replaced by well-defined arrayed or pooled libraries made from synthesized sequences. In addition to improving the efficiency and quality of cloned constructs and libraries, these synthetic sources of genetic sequences have enabled researchers to design and build defined libraries of elements that are not naturally occurring, such as barcoded sequences, novel promoters, CRISPR sgRNA, and shRNA.

    As one of the pioneers in creating libraries with synthetic oligonucleotides, Cellecta has developed a proficiency in producing high-quality libraries expressing short genetic elements, such as shRNA, peptides, enzyme active site elements, and most recently, sgRNA. While Cellecta uses standard proven cloning techniques to generate these libraries, there are many technical challenges that need to be overcome to fully produce libraries with representative, evenly distributed elements that are amenable to Next-Generation Sequencing (NGS) read-outs.

    Cellecta primarily focuses on producing:

    • CRISPR knockout, CRISPRa activation, and CRISPRi inhibition sgRNA libraries for genetic disruption and activation screens
    • pooled shRNA libraries for RNA interference (RNAi) screens
    • heterogenous barcode libraries for cell labeling

    We have also developed:

    • peptide expression libraries
    • miRNA libraries
    • promoter libraries
    • cDNA libraries with active domain variations

    Using the same basic approach described below, we can provide virtually any type of pooled library where the variable element can be defined within a couple-of-hundred-base sequence. Combining this with sophisticated cloning approaches enables us to produce a large variety of libraries, with barcodes if desired, in almost any vector.

    Contact us to explore your options for developing a pooled library for any type of screen.

    Library Construction Process

    Design and synthesize oligonucleotides for cloning.
    Oligonucleotides may be designed to encode shRNA or sgRNA (as described in the figure to the left), a peptide sequence, a portion of a larger protein, or may correspond to promoter variations, barcodes, or other genomic elements.
    Library cloning into the vector of choice.
    Once synthesized, oligonucleotides are cloned into the appropriate vector for the biological model and planned screening study. Cellecta has a selection of standard lentiviral vectors with different markers for typical libraries. However, we can make libraries in customer provide vectors, as well.
    Confirmation of library quality.
    After cloning, the distribution of cloned oligos in the pooled library is assessed using Next-Generation Sequencing (NGS).
    Packaging for libraries made in lentiviral vectors.
    As an optional service, Cellecta can package your library as VSV-g pseudotyped lentiviral particles. Various packaging scales are available.

    We also offer a variety of pre-made, off-the-shelf libraries for your CRISPR and RNAi experiments.

    Email us for more information.

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