Oligonucleotide and gene synthesis technology have largely made obsolete the more traditional techniques to clone genomic DNA and RNA isolated directly from organisms. Traditional cDNA, genomic, and other fragment libraries have become antiquated, replaced by well-defined arrayed or pooled libraries made from synthesized sequences. In addition to improving the efficiency and quality of cloned constructs and libraries, these synthetic sources of genetic sequences have enabled researchers to design and build defined libraries of elements that are not naturally occurring, such as barcoded sequences, novel promoters, CRISPR sgRNA, and shRNA.
As one of the pioneers in creating libraries with synthetic oligonucleotides, Cellecta has developed a proficiency in producing high-quality libraries expressing short genetic elements, such as shRNA, peptides, enzyme active site elements, and most recently, sgRNA. While Cellecta uses standard proven cloning techniques to generate these libraries, there are many technical challenges that need to be overcome to fully produce libraries with representative, evenly distributed elements that are amenable to Next-Generation Sequencing (NGS) read-outs.
We have also developed:
Using the same basic approach described below, we can provide virtually any type of pooled library where the variable element can be defined within a couple-of-hundred-base sequence. Combining this with sophisticated cloning approaches enables us to produce a large variety of libraries, with barcodes if desired, in almost any vector.
Contact us to explore your options for developing a pooled library for any type of screen.
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