Cellecta offers a range of inducible lentiviral vectors that enable modulation of both short RNA sequences (e.g., shRNA and sgRNA), and standard mRNA transcripts.
Cellecta provides inducible expression systems for both short RNA sequences, such as sgRNA and shRNA, using an expression cassette designed for RNA polymerase 3 with tet-responsive U6 or H1 promoters, as well as for inducible cDNA gene expression with the InDOXible tet-responsive RNA polymerase 2 promoter.
Inducible sgRNA and shRNA: The inducible U6 (or H1) promoter driving sgRNA/shRNA expression in our constructs is driven by RNA polymerase 3, which transcribes short RNA expression, like rRNA and tRNA. We developed the tet-inducible U6 promoter in-house at Cellecta.
Expression of cDNA: Most researchers are familiar with the tet-inducible expression system using a modified CMV promoter. Cellecta has developed an optimized Tet-Activated system regulated by the rtTA activator or tTA repressor.
Cellecta offers all the tet-inducible vector options as part of its sgRNA, shRNA, and cDNA custom lentiviral vector construction services.
(A) Inducible sgRNA and shRNA: Expression of sgRNA and shRNA is driven by the human U6 promoter. Cellecta has developed an inducible version of the U6 promoter using tet-operator control elements.
(B) Inducible cDNA Expression: Cellecta’s Tet-Responsive InDOXible™ System regulates expression of cDNA using hybrid activator/repressor proteins and a modified CMV promoter.
There are numerous applications where inducible gene expression can help advance research. Common applications include:
