CRISPR/RNAi Screening Services

Cellecta provides full CRISPR and RNAi screening services to help you identify the genes or genetic elements functionally regulating a biological response. We perform all steps for comprehensive pooled screens with your cell models. Tell us about the cells, phenotype, and function of the genes you are interested in identifying. Then, we will run the screen and provide you a report with comprehensive data indicating which gene(s)--when disrupted or perturbed by CRISPR sgRNA or RNAi shRNA--affect the response of interest. Cellecta can 

• Run genome-wide or targeted CRISPR knockout, CRISPRa, CRISPRi, and RNAi screens to meet your specific goals
• Screen one of our human or mouse genome-wide pooled libraries, or a custom library of any size, for any species
• For CRISPR screens, we make and test Cas9-expressing cell lines, which can be provided to you for future studies.
• Choose from a variety of FACS-based reporter and antibody selection options, in addition to more typical viability screens

We can provide all the services required to run most screens, including testing cell responses (e.g., kill curves, antibiotic sensitivity, etc.), cell engineering to make reporter cell lines and Cas9/dCas9-expressing lines, library construction and packaging, next-generation sequencing, and alignment to identify hits, and post-screening downstream analysis. Provide some details when you request a quote via the link above, and we will be happy to discuss your project with you.

More information on pooled genetic screens available here > 

Cellecta also offers single-cell CRISPR screening services. Learn more here >