Multiplex PCR for Gene Expression & Immune Receptor Profiling 

Powered by Cellecta’s proprietary primer design pipeline and optimized multiplex PCR workflow, our DriverMap™ Multiplex Targeted PCR Assays provide highly sensitive, targeted analysis of gene expression and immune receptor repertoires through proprietary multiplex PCR assays coupled with next-generation sequencing (NGS). Multiplex PCR amplification of specific targeted amplicons provides precise and reproducible quantification that enables researchers to efficiently quantify expression levels of tens of thousands of protein-coding genes and survey the diversity of hundreds of thousands of T- and B-cell receptors (TCRs/BCRs) clonotypes—even in limited samples.

Gene Expression Profiling with Unmatched Sensitivity and Reproducibility

The DriverMap Expression Profiling (EXP) Assays enable rapid and sensitive whole-transcriptome gene expression profiling from total RNA for biomarker discovery and differential expression studies. The assay is compatible with complex samples such as FFPE, dried blood, or xenografts. Since there is no requirement for rRNA depletion or mRNA enrichment, the DriverMap EXP assay is ideal when profiling small samples and limited amounts of cells. In fact, with low numbers of cells, including FNA samples, expression can be assayed directly in cell lysate.

Some applications:

Biomarker discovery and stratification for precision medicine
Gene expression profiling in preclinical models, patient-derived samples, and clinical trial cohorts
Transcriptomic analysis in single-cell or low-input RNA settings, including dried blood microsamples
Detection of clonal expansion, activation signatures, and treatment-associated shifts in TCR/BCR composition

Immuno-repertoire Profiling

The comprehensive range of DriverMap Adaptive Immune Receptor (AIR) Assays offer both RNA- and DNA-based profiling of T-cell and B-cell receptors (TCRs/BCRs) in bulk cell populations or individual cell-by-cell analysis. Assays provide sensitive detection of the CDR3 regions or paired-chain sequences for all TCR or BCR variants. Proprietary targeted primers enable rapid and accurate identification of clonotypes from isolated blood, PBMCs, bulk, fractionated, or sorted T-cells and B-cells; or full receptor chain pairing information.

Immune repertoire characterization in cancer immunotherapy, autoimmune diseases, and infectious disease research
Detection of shifts in TCR/BCR composition in response to vaccinations or antigen exposure
TCR/BCR chain pairing studies for neoantigen-targeting therapies and CAR-T development
Validation of immune checkpoint inhibitors and antibody therapeutics

Get Started

Whether you're working with whole blood, frozen tissue, PBMCs, or single cells, simply contact Cellecta to discuss your experimental goals. We offer off-the-shelf kits for genome-wide expression and immune receptor profiling, as well as contract profiling services for analysis of researcher-submitted samples. Further information is available below: