Catalog #: CVPRC

Custom Lentiviral cDNA Constructs

Lentiviral expression vectors are one of the most convenient and effective vehicles to introduce and stably express your gene of interest in almost any mammalian cell.

  • Obtain lentiviral vectors or pre-packaged lentiviral particles
  • Lentiviral vector for transduction and integration into the host cell genome
  • Stably passed onto progeny cells
  • A range of vectors with various fluorescent proteins genes and selection markers.

Let Cellecta clone your gene into one of our optimized lentiviral vectors and create a stable cell line for you.

Custom Lentiviral cDNA ConstructsWe can provide custom lentiviral constructs expressing any cDNA of interest as long as it is less than about 3.5 kb. There is a limit on the size of the construct because lentiviral plasmids cannot be packaged as lentiviral particles if they are too large. In addition to the expressed cDNA the portion of the plasmid packaged as a lentiviral particle contains elements to enable viral packaging, as well as some sort of selection. Often a fluorescent marker is desirable to enable identification of the cells that have been transduced with the lentiviral construct.

For our lentiviral cDNA construct service, you simply need to...

  • Provide the sequence of the cDNA you would like expressed
  • Let us know which fluorescent or selection markers you need
  • Select the desired promoter for your cells

With this information, we will...

  • synthesize the cDNA insert
  • clone the cDNA into the appropriate vector
  • sequence it to ensure proper cloning and plasmid design

We would be happy to discuss the options with you, or request a quote on-line.

Not finding what you are looking for? We are here to help.

Contact Us