For the standard CRISPR system, knockout efficiency is directly dependent on Cas9 expression levels. Cell lines stably expressing Cas9 are required for CRISPR screening with pooled sgRNA libraries. Transient expression of the Cas9 is not sufficient for pooled screening. For CRISPR screening purposes, it is important to use cells that express Cas9 well. Further, Cas9 expression levels definitely vary across different cell types, depending on the promoter used to drive Cas9 expression and the number of Cas9 copies per cell
Cellecta offers a variety of off-shelf, ready-to-use Cas9-Expressing Cell Lines for
Cellecta Pre-Made Cas9/dCas9 expressing cell lines are functionally validated using our CRISPR-Test assays (data is available in the product certificates) and are known to have high enough Cas9 activity to ensure successful CRISPR screens.
Don't waste time and resources on a CRISPR screen that will not work because your cells are not effectively expressing Cas9. Start your screen with confidence today.
If you don't find what you need, contact us about our Custom Cell Line Engineering service.
