DriverMap AIR is the only assay technology on the market that profiles the full or CDR3 repertoire of all T-cell receptor (TCR)--TRA, TRB, TRG and TRD--and B-cell receptor (BCR)--IGH, IGK and IKL--chains in an single-tube, single-day assay using multiplex PCR-NGS technology without the need of any additional specialized equipment. Cellecta offers AIR assays to profile RNA, DNA, or both. The simultaneous profiling enables the identification of antigen-activated clonotypes to provide even greater insight into the immune response.
How does the DriverMap AIR assay work?
Why Do I Need Information on Both T-Cell and B-Cell Receptor Repertoires?
Since T- and B-cells work synergistically in the adaptive immune response, we have designed an assay that profiles both T-cell receptor (TCR) and B-cell receptor (BCR) repertoires in a single convenient reaction. Separate assays specific for T- or B-cell chains are also available.
The DriverMap AIR-RNA assay provides the most sensitive detection of low-frequency rare TCR and BCR clonotypes and more comprehensive profiling when working with small samples and limited numbers of cells.
The DriverMap AIR DNA-based assay provides a more quantitative measurement of the number of cells with each CDR3-specific clonotype. This data enables the measurement of clonal expansion in T and B cells.
When information from both the AIR-DNA and AIR-RNA assays are combined, one can readily identify antigen-activated clonotypes. The heatmaps below (Fig. 1) show the activation of specific TRB and IGH clonotypes across multiple samples in metastatic tumor biopsies. For each sample (i.e., heatmap), data is normalized for RNA and DNA profiling; the green brackets show clonotypes present in RNA but not in DNA, indicating metastatic tumor-activated immune receptor clones
How is the DriverMap AIR Assay Different from other AIRR Assays?
Applications of BCR sequencing
Applications of TCR sequencing