DriverMap Adaptive Immune Receptor (AIR) TCR-BCR Profiling

DriverMap AIR is the only assay technology on the market that profiles the CDR3 repertoire of all T-cell receptor (TCR)--TRA, TRB, TRG and TRD--and B-cell receptor (BCR)--IGH, IGK and IKL--chains in an single-tube, single-day assay using multiplex PCR-NGS technology without the need of any additional specialized equipment.

  • Start from all immune sample types such as whole blood, PBMC, cancer biopsies, tissue samples, FFPE or dried blood microsamples.
  • Profile directly from immune cells (e.g.,100-50,000 FACS-sorted cells) without the need to isolate RNA.
  • Detect and accurately quantify more AIR clonotypes with Unique Molecular Identifiers (UMIs).
  • Amplify only functional TCR/BCR mRNA isoforms, without pseudogenes, ORFs or misarranged genes.
    • Only assay technology on the market that profiles the CDR3 repertoire of all T-cell receptor (TCR) and B-cell receptor (BCR) chains in an easy-to-run, single-tube, single-day assay using multiplex PCR-NGS technology without the need of any additional specialized equipment.

      While it is possible to purchase kits to profile the T- or B-cell chains separately, we recommend profiling both TCR and BCR repertoires together, since in most cases, T- and B-cells work synergistically in the adaptive immune response.

      • Start from various immune sample types such as whole blood, PBMC, cancer biopsies, tissue samples, FFPE or dried blood microsamples.
      • Profile directly from immune cells (e.g.,100-50,000 FACS-sorted cells) without the need to isolate RNA.
      • Detect and accurately quantify more AIR clonotypes with Unique Molecular Identifiers (UMIs)
      • Amplify only functional TCR/BCR mRNA isoforms, without pseudogenes, ORFs or misarranged genes.
      • DriverMap™ Multiplex PCR technology uses gene-specific primers which significantly reduce the level of non-specific binding and primer-dimer amplification products, and are designed to target only expressed TCR/BCR mRNA isoforms.
      • Unique Molecular Identifiers (UMIs) facilitate accurate quantitation of the copy number of cDNA molecules in amplification steps, as well as detection of low abundance clonotypes and correction of amplification biases and sequencing errors.
      • Dual-index amplicon labeling strategy minimizes index hopping during NGS allowing for comprehensive readouts.
      • Full profiles of the antigen-recognition CDR3 region enable assessment of CDR3 length distribution, V(D)J segment usage, isotype composition for BCRs, somatic mutations, and similar characteristics with immune receptor profiling software such as MiXCR (MiLabs).
    • Applications of BCR sequencing

      • Identify broadly neutralizing antibodies (BNAbs) and map Ig-seq datasets to known antibody structures for antibody and vaccine development.
      • Track B-cell migration and development patterns
      • Find clinical markers of autoimmune diseases such as multiple sclerosis, rheumatoid arthritis and cancers (e.g. B-cell lymphoma)
      • Contrast naïve and antigenically challenged datasets for understanding antibody maturation

      Applications of TCR sequencing

      • Track T-cell clonality and diversity for mechanism of action of immune checkpoint inhibitors for immunotherapies
      • Assess TCR overlap between repertoires to define spatial and temporal heterogeneity of the anti-tumoral immune response
      • Analyze TCR sequence and structure to annotate antigenic specificity for developing personalized cellular immunotherapies

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