Catalog #: CVSHC

Custom shRNA Constructs

Cellecta provides complete services for construction of custom lentiviral constructs expressing shRNA targeting any transcript.

  • Choose constitutive or inducible version of H1 or U6 shRNA promoters
  • Select GFP, RFP, PuroR, BleoR, NeoR, or Hygro-HK markers
  • Obtain constructs as plasmid or packaged lentiviral particles

We can provide 3 to 5 shRNA constructs targeting a specific human or mouse gene. These are constructed based on design parameters we use in creating our libraries.

Custom shRNA ConstructsTo make an lentiviral shRNA construct, we only need to know the RefSeq number. We typically design and clone 3-5 constructs that target your transcript. Our proprietary shRNA design delivers approximately 70% shRNAs that generate >70% transcript knockdown in standard cell lines as measured by qRT-PCR so it is likely that at least 2 of 3 or 3 of 5 constructs will be effective.

We make the inserst, clone them, and sequence the constructs to verify correct construction. You receive several of our best-designed constructs to test and characterize to see which best matches your experimental needs. We can provide the plasmid constructs, or you have the option to also package the constructs as ready-to-transduce lentiviral particles.

We do not guarantee this level for any specific target, and the percentage of highly effective shRNAs will vary from target to target.

 

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