Cell Line Engineering

Everything You Need to Build or Obtain the Cell Line You Want

Overview

Cellecta offers comprehensive cell engineering solutions, whether you want to outsource your project or prefer to engineer your own lines. We provide complete, end-to-end services for creating custom engineered cell lines, as well as a robust catalog of high-quality lentiviral vectors, pre-made Cas9 lines, packaging reagents, and ready-to-use constructs. With options for knockout, knock-in, activation, knockdown, overexpression, and reporter tagging, Cellecta is your partner in cell model development.

Applications

• CRISPR/Cas9 and RNAi knockout/knockdown cell line development
• Stable overexpression or inducible transgene models
• Multiplexed or dual-effector genetic systems
• Pooled or clonal format screening models

Contract Cell Engineering Services

Cellecta offers complete contract services for generating engineered cell models tailored to your research goals. We work collaboratively with you to design and deliver CRISPR, RNAi, or transgene-based modifications in your chosen cell type. We provide expert project guidance, sequence verification, and functional validation for cell engineering projects. Every project is customized — from vector design to final QC — ensuring your engineered line performs exactly as intended. Whether you're creating models for drug screening, target validation, pathway studies, or viral infectivity, we provide:

• Knockout, knock-in, overexpression, reporter, and dual-effector engineering services
• Zero-footprint CRISPR editing via non-integrating Cas9/sgRNA delivery
• Development of transcriptional and functional reporter cells
• Experience with hard-to-transduce lines and primary cells
• Custom Cas9 or dCas9 variant cell lines with verified activity
• High-efficiency constructs with fluorescent or antibiotic selectable markers
• Cell lines delivered as validated clonal isolates or stable pools

Build Your Own Cells with Our Tools

Do you prefer to engineer cells in your own lab? Cellecta provides a wide range of customizable constructs, premade cell lines, and other related reagents to help you project:

• Custom Lentiviral cDNA Expression Vectors
  • Constitutive or inducible
  • Packaged or plasmid-only
  • Wide range of markers and fluorescent reporters
• Custom sgRNA and shRNA Vectors
  • CRISPR, CRISPRa, and CRISPRi constructs
  • sgRNA-only, dual-sgRNA
  • Inducible designs
  • Pre-made verified non-targeting and lethal controls
• High-efficiency packaging reagents for lentiviral production
• Pre-Made Cas9/dCas9 Cell Lines: Validated and ready-to-use for immediate transduction

Get Started—See Links Below for More Information

1. Custom Cas9 and dCas9 Variant Cell Lines
2. Custom Engineered Cell Lines
3. Virus Receptor Cell Line Engineering Service (e.g., ACE2, DPP4 Expressing Cells)
4. Cas9 and dCas Expressing Cell Lines

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