DriverMap Single-Cell AIR TCR-BCR Assay Kits

Obtain the full-length sequence of both chains of T-cell (TCR) or B-cell receptors (BCR) from flow-sorted or pipetted T or B cells with the DriverMap Single-Cell Adaptive Immune Receptor (scAIR) TCR-BCR assays. These consist of a variety of easy-to-use, 96-well, plate-based RT-PCR kit configurations that help you obtain:

• Full-length sequences of both alpha/beta or IGH/IGK/IGL receptor chains: Obtain complete coding region sequences for human, mouse, and mouse/human hybrid T-cell (TRA-TRB) or B-cell(IGH-IGK-IGL) receptors.
• Simple, streamlined protocol: Sort cells into an indexed 96-well plate with RT primers, hybridize, then pool samples into a single reaction tube. Perform RT-PCR and next-generation sequencing (NGS), scTCR-Seq or scBCR-Seq. No RNA extraction, specialized equipment, or complex barcoding required.
• Characterize full receptors from single cells or 1000s of cells: Two workflow options--the first for 96 flow-sorted individual cells per plate; and the second for up to ~10,000 cells (~100cells/well) to thoroughly characterize each receptor in small numbers of cells, or extract large numbers of abundant receptor chain pairs in larger cell populations.
• Cost-effective: Economical analysis, starting from approximately 10 cents per cell.
• Integrated bioinformatics analysis pipeline with MiXCR software on Platforma (MiLaboratories, Inc.) to process FASTQ files, obtain clonotype alignment and chain-pair information, and optionally, for human samples, analyze expression of key marker genes that show the immunophenotype of each cell. Other open-source tools may also be used for further data processing.

TheDriverMap ™scAIRTCR/BCRKits include all the enzymes, reagents, and primers to preparesamples for profilingusing next-generation sequencing (NGS): scTCR-Seq or scBCR-Seq.

Fig 1: Plate image of DriverMap scAIR TCR-Mark36 Immunophenotyping Kit data from flow-sorted 96 cells/plate. The table shows that 65% of the cells detected had a TCR-αβ chain pair.  

 

Fig 2: DriverMap scAIR Chain Pairing Assay Workflowfor TCR/BCR paired-chain analysis from flow-sorted or pipetted single cells or multiple cells per well.  The hybridization plate contains a combination of forward gene-specific primers (Fwd GSPs) targeting the FR1 region and barcoded reverse gene-specific primers (Rev GSPs) targeting the C region of TCR/BCRs. RevGSPs have 96 cell/well-specific barcodes (Cell-BC) and validator barcodes (VBCs) to facilitate quantitative analysis of paired receptor chains. Before cDNA synthesis, all cells may be pooled into a single tube for easy handling. Anchor 1 and Anchor 2 are sequences of universal primers used in the first amplification step. P5-UD1-Anchor1 and P7-UD2-Anchor2 are primers with Illumina UD indexes used in the second PCR step. The resulting NGS data provides chain pairing information and clonotype abundance levels for each gene. 

 

 

DM-SCAIR-HTR	DriverMap™ scAIR TCR Chain Profiling Kit (Human)	1 kit (enough for eight 96-well plates)
DM-SCAIR-MTR	DriverMap™scAIR TCR Chain Profiling Kit (Mouse)	1 kit (enough for eight 96-well plates)
DM-SCAIR-HBR	DriverMap™scAIR BCR Chain Profiling Kit (Human)	1 kit (enough for eight 96-well plates)
DM-SCAIR-MBR	DriverMap™scAIR BCR Chain Profiling Kit (Mouse)	1 kit (enough for eight 96-well plates)
DM-SCAIR-HTR-M36	DriverMap™scAIR TCR-Mark36 Immunophenotyping Kit (Human)	1 kit (enough for eight 96-well plates)
DM-SCAIR-HBR-M30	DriverMap™scAIR BCR-Mark30 Immunophenotyping Kit (Human)	1 kit (enough for eight 96-well plates)
DM-SCAIR-HM-TR	DriverMap™scAIR TCR Chain Profiling Kit (Hybrid Human-Mouse)	1 kit (enough for eight 96-well plates)
DM-SCAIR-HM-BR	DriverMap™scAIR BCR Chain Profiling Kit (Hybrid Human-Mouse)	1 kit (enough for eight 96-well plates)

The DriverMap scAIR assay is also available as a service, where you would send us your flow-sorted cells on a 96-well plate(s), and Cellecta will return a comprehensive analysis report. More information on the service here >

For more information on clonal repertoire diversity and more, you may want to consider the DriverMap AIR assay for bulk samples. Information on the DriverMap bulk AIR assay is available here >

Manual and Product Resources

• Manual: DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay (Human, Mouse, Hybrid Human-Mouse) - V2 

• Manual: DriverMap™ Single-cell AIR TCR-BCR Immunophenotyping Assay (Human) - V2 

• Technology Guide: DriverMap AIR Technology Guide for an introduction to AIR technology and for recommendations on designing an AIR profiling experiment

• Product Flyers and other Resources:

  • Flyer: Comprehensive Adaptive Immune Receptor Profiling for All Immune Sample Types

  • Flyer: Synthetic Spike-in Controls for Immune Repertoire Profiling

  • Brochure: DriverMap Technology Solution

  • Poster: A novel 96-well plate-based FACS-sorted single-cell approach allows the identification of immune receptor chain pairing & immunophenotyping of T or B cells

  • Video: Immunophenotyping of T and B-Cell Receptors; by Alex Chenchik, Cellecta, Inc

  • New! Video: A guide for adaptive immune receptor profiling & characterization of antigen-induced clonotypes(18:46 min) Alex Chenchik, Cellecta, Inc

  • New! Video: Qualitative assessment of the quality of immune response by analyzing B- and T-cell clonotypes(18:31 min) Andrei Gudkov, Roswell Park Cancer Center

 

• Tech Notes / Blogs

• Comparing DNA vs. RNA Samples for Immune Repertoi