DriverMap™ Single-Cell AIR TCR-BCR Assay Kits (Human / Mouse or Human-Mouse Hybrid RNA)

$4,850.00

U.S. List Price more info: Prices shown reflect U.S. list pricing for orders shipped to U.S. addresses. Non-U.S. pricing may vary depending on geography. Please contact orders@cellecta.com for a quote or check our distributor page to see if Cellecta has an authorized reseller in your region.

Product Information

Obtain the full-length sequence of both chains of T-cell (TCR) or B-cell receptors (BCR) from flow-sorted or pipetted T- or B-cells with the DriverMap Single-Cell Adaptive Immune Receptor (scAIR) TCR-BCR assays. These consist of a variety of easy-to-use, 96-well, plate-based RT-PCR kit configurations

Full-length sequences of both TCR alpha/beta or BCR IGH/IGK/IGL receptor chains: With the DriverMap scAIR Chain Pairing Reagent Kit, you obtain complete coding region sequences for human, mouse, and mouse/human hybrid T-cell (TRA-TRB) or B-cell(IGH-IGK-IGL) receptors.
- Additionally, with the DriverMap scAIR Immunophenotyping Kits, you obtain immunophenotyping information from 36 T-cell or 30 B-cell expression marker genes for further info on cell state
Simple, streamlined protocol: Sort cells into an indexed 96-well plate with RT primers, hybridize, then pool samples into a single reaction tube. Perform RT-PCR and next-generation sequencing (NGS), scTCR-Seq or scBCR-Seq. No RNA extraction, specialized equipment, or complex barcoding required.
Characterize full receptors from single cells or 1000s of cells: Two workflow options--the first for 96 flow-sorted individual cells per plate; and the second for up to ~10,000 cells (~100cells/well) to thoroughly characterize each receptor in small numbers of cells, or extract large numbers of abundant receptor chain pairs in larger cell populations.
Cost-effective protocols: Economical price point, starting from approximately 10 cents per cell.
Integrated bioinformatics analysis pipeline with MiXCR software on Platforma (MiLaboratories, Inc.) to process FASTQ files, obtain clonotype alignment and chain-pair information, and, optionally, for human samples, analyze expression of key marker genes that show the immunophenotype of each cell. Other open-source tools may also be used for further data processing.

DriverMap scAIR TCR/BCR Reagent Kits include all the enzymes, reagents, and primers to go from samples to NGS-ready libraries. A separate Universal Unique Dual-Index NGS plate is required for next-generation sequencing (NGS) run: scTCR-Seq or scBCR-Seq.

Fig 1. Plate image of DriverMap scAIR TCR-Mark36 Immunophenotyping Kit data from flow-sorted 96 cells/plate. The table shows that 65% of the cells detected had a TCR-αβ chain pair.

Cellecta DriverMap Single-Cell TCR-BCR Profiling Assay Workflow

Fig 2: DriverMap scAIR Chain Pairing Assay Workflow for TCR/BCR paired-chain analysis from flow-sorted or pipetted single cells or multiple cells per well. The hybridization plate contains a combination of forward gene-specific primers (Fwd GSPs) targeting the FR1 region and barcoded reverse gene-specific primers (Rev GSPs) targeting the C region of TCR/BCRs. RevGSPs have 96 cell/well-specific barcodes (Cell-BC) and validator barcodes (VBCs) to facilitate quantitative analysis of paired receptor chains. Before cDNA synthesis, all cells may be pooled into a single tube for easy handling. Anchor 1 and Anchor 2 are sequences of universal primers used in the first amplification step. P5-UD1-Anchor1 and P7-UD2-Anchor2 are primers with Illumina UD indexes used in the second PCR step. The resulting NGS data provide chain-pairing information and clonotype abundance levels for each gene.

Workflow Requirements

Each DriverMap™ scAIR TCR-BCR Assay Reagent Kit includes all specialized enzymes, primers, and reagents required for library preparation; however, to run the libraries on Illumina® next-generation sequencing (NGS) platforms, a compatibleUniversal Unique Dual Index (UDI) NGS Index Platemust be purchased separately as a required component of the workflow.

Purchase both a 1) DriverMap scAIR Assay Reagent Kit and a 2) Universal Unique Dual-Index (UDI) NGS Plate

Catalog #	DriverMap scAIR Assay Reagent Kits	Quantity
DM-SCAIR-HTR-B1	DriverMap™ scAIR TCR Chain-Pair Profiling Kit (Human)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-HBR-B1	DriverMap™scAIR BCR Chain-Pair Profiling Kit (Human)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-MTR-B1	DriverMap™scAIR TCR Chain-Pair Profiling Kit (Mouse)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-MBR-B1	DriverMap™scAIR BCR Chain-Pair Profiling Kit (Mouse)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-HM-TR-B1	DriverMap™scAIR TCR Chain-Pair Profiling Kit (Hybrid Human-Mouse)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-HM-BR-B1	DriverMap™scAIR BCR Chain-Pair Profiling Kit (Hybrid Human-Mouse)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-HTR-M36-B1	DriverMap™scAIR TCR-Mark36 Chain-Pair & Immunophenotyping Kit (Human)	1 kit (enough for eight 96-well plates)	Order Now >
DM-SCAIR-HBR-M30-B1	DriverMap™scAIR BCR-Mark30 Chain-Pair & Immunophenotyping Kit (Human)	1 kit (enough for eight 96-well plates)	Order Now >

Required Product

Catalog #	Universal Unique Dual-Index (UDI) NGS Plates	Quantity
DM-NGS-SetA-B2	Unique Dual Index Set A for DriverMap EXP or AIR (1-96)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetB-B2	Unique Dual Index Set B for DriverMap EXP or AIR (97-192)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetC-B2	Unique Dual Index Set C for DriverMap EXP or AIR (193-288)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetD-B2	Unique Dual Index Set D for DriverMap EXP or AIR (289-384)	1x 96-Well Plate (4 rxn/well)	Order Now >

The DriverMap scAIR assay is also available as a service, where you would send us your flow-sorted cells on a 96-well plate(s), and Cellecta will return a comprehensive analysis report. More information on the service here >

For more information on clonal repertoire diversity and more, you may want to consider the DriverMap AIR assay for bulk samples. Information on the DriverMap bulk AIR assay is available here >

Technology Guide: DriverMap AIR Technology Guide for an introduction to AIR technology and for recommendations on designing an AIR profiling experiment