DriverMap™ AIR TCR-BCR Profiling Kit (Human DNA)

$1,450.00

U.S. List Price more info: Prices shown reflect U.S. list pricing for orders shipped to U.S. addresses. Non-U.S. pricing may vary depending on geography. Please contact orders@cellecta.com for a quote or check our distributor page to see if Cellecta has an authorized reseller in your region.

Product Information

Easy-to-run, single-day assay that uses multiplex PCR-NGS technology to profile T-cell receptor (TCR) or B-cell receptor (BCR) repertoire present in human DNA. The DriverMap AIR DNA assay measures the frequency of CDR3 clonotypes to quantify the clonal frequency of T- and B-cells.

Assay primers contain Validator Barcodes (VBCs) to enable accurate quantitation of each clonotype.
Sensitive profiling of samples with low lymphocyte content (e.g. FFPE, tumor biopsy, achieved specimens) where DNA is more stable than RNA.
The AIR Profiling Reagent kit and the required universal unique dual index (UDI) NGS kit include all the enzymes, reagents, and primers to prepare 24 or 96 samples, ready for multiplex Illumina NGS runs, from DNA starting material. No additional specialized equipment required.
Parallel runs of DriverMap AIR DNA Assay with DriverMap AIR RNA Assay reveal potential disease-associated, activated CDR3 clonotypes

DriverMap™ AIR TCR samples (Human DNA samples) - Kits

Fig 1: Mean clonotype counts for four TCR chains from different DNA sample inputs.

Workflow Requirements

Each DriverMap™ AIR TCR-BCR Profiling Reagent Kit includes all specialized enzymes, primers, and reagents required for target enrichment and library preparation; however, to run the libraries on Illumina® next-generation sequencing (NGS) platforms, a compatibleUniversal Unique Dual Index (UDI) NGS Index Platemust be purchased separately as a required component of the workflow.

Purchase both

1) DriverMap AIR Reagent Kit, AND

2) Universal Unique Dual Index (UDI) NGS Plate

Catalog #	DriverMap AIR Reagent Kit	Quantity
DMAIR2-HBD-24-B1	DriverMap™ AIR DNA CDR3 BCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HBD-96-B1	DriverMap™ AIR DNA CDR3 BCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >
DMAIR2-HTD-24-B1	DriverMap™ AIR DNA CDR3 TCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HTD-96-B1	DriverMap™ AIR DNA CDR3 TCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >

Required Product for NGS Run

Catalog #	Universal Unique Dual Index (UDI) NGS Plate	Quantity
DM-NGS-SetA-B2	Unique Dual Index Set A for DriverMap EXP or AIR (1-96)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetB-B2	Unique Dual Index Set B for DriverMap EXP or AIR (97-192)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetC-B2	Unique Dual Index Set C for DriverMap EXP or AIR (193-288)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetD-B2	Unique Dual Index Set D for DriverMap EXP or AIR (289-384)	1x 96-Well Plate (4 rxn/well)	Order Now >

Manual: DriverMap AIR DNA Profiling Kit Manual

Technology Guide: DriverMap AIR Technology Guide for an introduction to AIR technology and for recommendations on designing an AIR profiling experiment