DriverMap™ Human Genome-Wide Expression Profiling Kit, V3

The DriverMap™ Targeted RNA Sequencing Expression Profiling (EXP) Assay is a convenient single-tube RT-PCR / Next-Generation sequencing (NGS) assay that robustly measures expression levels of 19,000 human protein-coding genes from ng levels of total RNA from blood, cells, tissue, even FFPE samples.  The DriverMap EXP kit includes all the enzymes, reagents, and primers to prepare NGS-ready libraries from total RNA. Open-source Salmon software is used to quantify expression levels for each gene. Further downstream analysis may be carried out using a variety of differential expression tools.

The DriverMap Targeted RNA-Seq EXP Assay is available in off-the-shelf kit formats for blood, tissue, cells or from dried blood microsamples, and as a service.

• DriverMap EXP Human Genome-Wide 19K Profiling Kit (starting material: blood, tissue or cells, 24- or 96-sample kits)
• DriverMap EXP Human Genome-Wide 19K Dried Blood Microsample Profiling Kit (24- or 96-sample kits) 
  
  • This innovative solution for transcriptomic analysis of small volumes of whole blood (e.g. 30 μl) is a convenient and reliable way of obtaining high-throughput gene expression data for monitoring gene regulation and expression for biomarker and drug discovery applications. With this method, easy sample collection from remote locations without access to traditional blood collection facilities is possible.
  • The DriverMap EXP Dried Blood kit contains additional reagents for RNA extraction from the Mitra tips as well as all the other primers and reagents required for NGS library prep included in the original DriverMap EXP Profiling kit.
  • To use the DriverMap EXP Dried Blood protocol, you will need to order the Neoteryx Mitra tips and GenTegra GTR-NEO Active Chemical Protection of RNA solution separately from the Neoteryx site according to the table below:

The figure above shows reproducible and sensitive expression profiling with >90% overlap in gene expression profiles obtained from two different donors whose blood was extracted using standard blood collection tubes (400 ul) and Mitra tips (30 ul), with strong correlation (r=0.94) between results.

Manual and Product Documentation

• Manual: DriverMap Targeted Expression Profiling Kits (for blood, cells, tissue)
• Manual: DriverMap Targeted Expression (EXP) Dried Blood Microsampling Profiling Kits (for dried blood samples obtained using the Neoteryx Mitra device)
• Brochure: DriverMap™ Human Genome-Wide Expression Profiling Solution
• Reference: Publication: Tarca et al., Scientific Reports, (2019) 9:848 Targeted expression profling by RNA-Seq improves detection of cellular dynamics during pregnancy and identifes a role for T cells in term parturition
• Microsampling Poster: Microsampling for RNA Biomarker Profiling in Blood
• Gene list: Gene List DriverMap Human Genome-Wide v3

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