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Next-Gen Sequencing of CRISPR/RNAi and Barcode Libraries

Get an NGS Analysis kit that has all the primers and reagents to prepare sgRNA, shRNA, or barcodes from pooled lentiviral libraries for sequencing, or send Cellecta your samples and we will prep and sequence them for you.

  • Amplify and sequence barcodes or sgRNA from genomic DNA samples
  • Get enumerated shRNA, sgRNA, or barcode counts for downstream analysis
  • Buy complete NGS analysis kits with all reagents and primers needed, or use Cellecta's sequencing service

  • Pooled lentiviral CRISPR, shRNA, and barcode libraries require targeted PCR amplification of the effectors from genomic DNA after screens. Cellecta offers kits with complete reagents and primers to generate sequencing-ready samples from genomic DNA isolated from pooled cells. Software included with the kit enables rapid and convenient analysis of the sequencing. The program demultiplexes indexed samples and aligns sgRNA reads from the Illumina NGS FASTQ files. The output is a spreadsheet with the counts of each sgRNA in each sample.

    Alternatively, we provide complete Next-Gen Sequencing (NGS) and analysis services for researchers running their own screens or cell tracking experiments with pooled libraries.

    Next-Gen Sequencing CRISPR/RNAi Libraries

  • Use of a pooled lentiviral CRISPR, shRNA, or barcode library requires amplification and NGS analysis from genomic DNA. Our service includes DNA prep, sequencing, and analysis so you receive a spreadsheet of counts per guide/sample.

    Cellecta NGS Analysis Kits provide enzymes, reagents, and indexed primers for multiplex sequencing. Kits support 6–48 samples, with optional indexing expansion.

    You can also send samples to Cellecta for full-service processing and analysis.

    NGS cassette

  • After pooled screens, genomic DNA is sequenced to quantify sgRNA, shRNA, or barcode abundance. This identifies enriched or depleted elements.

    Common use cases include identifying essential genes, cancer vulnerabilities, and tracking clonal populations in barcoding studies.

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