Use of a pooled lentiviral CRISPR, shRNA, or barcode library in an experiment requires amplification and next-generation sequencing (NGS) analysis of the barcodes or guides from genomic DNA isolated from cells transduced with the library. Our service includes the DNA preparation, sequencing run, and demultiplexing/alignment, so you receive a spreadsheet with reads/guide/sample for each element in the library, with optional DNA isolation from cells or tissue samples.
Cellecta NGS Analysis Kits provide the enzymes, reagents, and 15 indexed amplification primers used to prepare sequencing libraries for multiplex NGS analysis. Primers are specific to each library/vector combination, so you simply need to choose a kit appropriate for the library you are using. Each kit contains sufficient primers and reagents for 6-48 samples (48 preps of 50 ug each, 12 multiplex). Supplementary indexing primers are also available, which increase multiplexing capacity to 24 samples.
Alternatively, you can send Cellecta cells, tissues, or genomic DNA and have us prepare, sequence and analyze the samples for you.