Catalog #: CANA

Sequencing Service for Samples from Genetic Screens

Get results from gene screens with pooled CRISPR, shRNA, and barcode libraries faster by letting Cellecta do the NGS prep, sequencing, and analysis.

  • Send us cells or DNA and get a report with the number of reads per guide, hairpin, or barcode
  • Rapid turnaround time
  • Able to sequence all Cellecta libraries and a range of other pooled lentiviral libraries, such as Brunello, GecKO, etc.

You provide Cellecta with frozen cell pellets, tissue or genomic DNA and we do the rest!

Sequencing Service for Samples from Genetic ScreensIf you are running your own pooled lentiviral gene screens with CRISPR sgRNA, RNAi hairpin, or cell barcode labeling library just provide harvested cells or genomic DNA for each time point or treatment condition (one sample), and we will provide you with a spreadsheet enumerating the read number for each of the library elements. Our Genetic Screens Sequencing services will...

  • Extracts genomic DNA
  • Amplifies barcodes or sgRNA
  • Performs NGS on the Illumina NextSeq or HiSeq
  • Enumerates shRNA, sgRNA, or barcode counts from raw sequencing data

Additional bioinformatics and pathway analysis available on request.

Sequencing is provided not only for Cellecta libraries but also similar non-Cellecta CRISPR libraries, such as GeCKO, Brunello, Brie library, etc. We have extensive experience in designing primer and running NGS on a large variety of pooled libraries for various types of genetic screens.

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