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Oligonucleotide CRISPR, shRNA, and Barcode Libraries 

High-quality lentiviral and retroviral oligonucleotide libraries expressing individual or combinations of sgRNA, shRNA, or barcodes for functional screening, lineage tracing, and other perturbation and cell analysis applications.

  • Overview

    Cellecta specializes in the design, synthesis, and delivery of custom and off-the-shelf oligo libraries—supporting CRISPR knockout, RNAi knockdown, barcode lineage tracing, and other high-throughput functional genomics applications. Our unique cloning and amplification methods maintain complexity and ensure even representation across diverse libraries. Whether you need a genome-wide library or a focused pool for a specific pathway, we deliver rapid, NGS-verified solutions.

  • Applications

    • CRISPR knockout, loss-of-function or gain-of-function screening
    • shRNA knockdown studies
    • Barcode lineage tracing
    • Pooled screening for gene-drug interactions
    • Clonal cell tracking

  • Our Expertise and Differentiators

    • Custom and Pre-Made Libraries — order from our ready-to-use portfolio or design your own library with optimized guides or barcodes.
    • High-Fidelity Synthesis and Cloning — proprietary methods and high complexity cloning procedures minimize bias, preserve diversity, and support efficient cloning of 10 complexity pools.
    • Designed for Successful Lentiviral Delivery — libraries are cloned in standard lentiviral vectors and can be packaged by Cellecta or in your lab. Retroviral library backbones are also available.
    • NGS-Verified and Ready to Use — every batch is sequence-verified and delivered as plasmid DNA, RNA, or packaged lentiviral particles.
    • Support for Complex Screens and Cell Tracking — Cellecta provides protocols, tools for screening, bioinformatics analysis, and technical support to ensure success with CRISPR, RNAi, and barcode libraries.

  • Get Started

    1. Need a custom library?
    2. Off-the-shelf CRISPR Knockout Libraries
    3. Off-the-shelf CRISPRa and CRISPRi Libraries
    4. Off-the-shelf shRNA Knockdown Libraries
    5. CRISPR-Seq and Barcoded sgRNA Libraries
    6. Want us to run a CRISPR/shRNA Library screen?

  • FAQs