Oligonucleotide CRISPR, shRNA, and Barcode Libraries 

High-quality lentiviral and retroviral oligonucleotide libraries expressing individual or combinations of sgRNA, shRNA, or barcodes for functional screening, lineage tracing, and other perturbation and cell analysis applications.

Overview

Cellecta specializes in the design, synthesis, and delivery of custom and off-the-shelf oligo libraries—supporting CRISPR knockout, RNAi knockdown, barcode lineage tracing, and other high-throughput functional genomics applications. Our unique cloning and amplification methods maintain complexity and ensure even representation across diverse libraries. Whether you need a genome-wide library or a focused pool for a specific pathway, we deliver rapid, NGS-verified solutions.

Applications

• CRISPR knockout, loss-of-function or gain-of-function screening
• shRNA knockdown studies
• Barcode lineage tracing
• Pooled screening for gene-drug interactions
• Clonal cell tracking

Our Expertise and Differentiators

• Custom and Pre-Made Libraries — order from our ready-to-use portfolio or design your own library with optimized guides or barcodes.
• High-Fidelity Synthesis and Cloning — proprietary methods and high complexity cloning procedures minimize bias, preserve diversity, and support efficient cloning of 10 complexity pools.
• Designed for Successful Lentiviral Delivery — libraries are cloned in standard lentiviral vectors and can be packaged by Cellecta or in your lab. Retroviral library backbones are also available.
• NGS-Verified and Ready to Use — every batch is sequence-verified and delivered as plasmid DNA, RNA, or packaged lentiviral particles.
• Support for Complex Screens and Cell Tracking — Cellecta provides protocols, tools for screening, bioinformatics analysis, and technical support to ensure success with CRISPR, RNAi, and barcode libraries.

Get Started

1. Need a custom library?
2. Off-the-shelf CRISPR Knockout Libraries
3. Off-the-shelf CRISPRa and CRISPRi Libraries
4. Off-the-shelf shRNA Knockdown Libraries
5. CRISPR-Seq and Barcoded sgRNA Libraries
6. Want us to run a CRISPR/shRNA Library screen?