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Home / CRISP-Seq Barcoded sgRNA Libraries (Perturb-Seq, CROP-Seq) / Single-Cell CRISPR Screening Service: Perturb-Seq, CRISP-Seq, CROP-Seq etc.
Single-Cell CRISPR Screening Service: Perturb-Seq, CRISP-Seq, CROP-Seq etc.

Family: CRISP-Seq Barcoded sgRNA Libraries (Perturb-Seq, CROP-Seq), CRISPR Knockout Pooled Lentiviral sgRNA Libraries, Functional Genetic Screens with Pooled CRISPR / shRNA Libraries , Functional Genomics Contract Screens

Catalog #: CRGC-PS

Single-Cell CRISPR Screening Service: Perturb-Seq, CRISP-Seq, CROP-Seq etc.

With Cellecta’s Single-Cell CRISPR Screening Service (aka Perturb-Seq, CRISPR-Seq, CROP-Seq, etc.) you can study the effect of CRISPR-based perturbations on mRNA expression in many thousands of cells using single-cell RNA sequencing (scRNA-Seq) on the 10X Chromium system.  This method can provide important insights into the transcriptional response to genetic mutations and other perturbations affecting gene function.

  • Investigate transcriptional changes associated with 100+ genes in a single experiment
  • Combine pooled CRISPR knockout (KO), CRISPR inhibition (CRISPRi) or CRISPR activation (CRISPRa) screening with single-cell expression analysis
  • Identify genes affecting gene and pathway activation that are missed with conventional arrayed screens

General Information

Cellecta offers its Single-Cell CRISPR Screening Service on the 10X Chromium Controller Platform, which allows for screening of multiple genes in a single experiment and enables the identification of transcriptional profiles linked to specific gene perturbations.

We provide all services required to run most screens on-site, including construction and packaging of custom pooled sgRNA libraries, single-cell RNA-Seq on our in-house 10X Chromium and Illumina sequencing instruments, read alignment to identify hits, and post-screening analysis.

Single-Cell CRISPR Screening workflow

  • Step 1: Clone sgRNA library
  • Step 2: Cells transduced with pooled sgRNA libraries are loaded in the 10X reaction
  • Step 3: Beads are co-encapsulated with unique barcodes and cells are dispersed in discrete droplets
  • Step 4: The sgRNA and mRNAs labeled with bead barcodes are used to construct libraries, and running scRNA-Seq, single-cell transcriptional profiles are generated
  • Step 5: Changes in gene expression associated with specific sgRNAs are linked to gene-specific knockouts

For an overview of Cellecta's other functional genomics offerings, see CRISPR & RNAi Functional Genomics Capabilities

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