CloneTracker™ XP-CRISPR Mouse 27 Anti-Cancer CRISPR-Barcode-3 Library - RFP-Puro

$2,250.00

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Mouse sgRNA library incorporating clonal barcodes adjacent to the gRNA sequences. The library is designed for CRISP-Seq (aka Perturb-Seq) screens. In cells transduced with these libraries, researchers can detect both the clonal barcodes and the sgRNA effector with RNA Sequencing so that changes in gene expression can be correlated with specific disruptions of particular genes.

Cellecta currently offers two CloneTracker XP CRISPR Knockout libraries in the pRSGScribe1cb-EFS-TagRFP-2A-Puro-U6-sgRNA-BC30-3LTR vector, one targeting 27 human anti-cancer genes and one targeting the 27 corresponding mouse homologs. There are 20 sgRNAs to each gene designed to target functional domains.

Gene List: CloneTracker XP-CRISPR Mouse 27 Barcode-3' Library
Manual: CRISPR Pooled Lentiviral sgRNA Libraries Manual
References:

* Sequences are available in FASTA (.txt), GenBank (.gb), and SnapGene (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us. To download .txt and .gb files, right click on the link and Save As.