Family: DriverMap Adaptive Immune Receptor (AIR) TCR-BCR Profiling, DriverMap AIR TCR/BCR Profiling: Multiplex RT-PCR based TCR- or BCR-Sequencing of Bulk or Single-Cell Samples

DriverMap™ Single-Cell AIR T-Cell and B-Cell Receptor Profiling Service (to Obtain Chain-Pairing Info)

Cellecta offers a simple and efficient full-length TCR or BCR chain-pairing identification service starting from

flow-sorted individualT- and B-cells, OR from
small cell pools of ~100 cells/wellin a 96-well plate format

avoiding the workflow complexity and high cost of microfluidics-based, single-cell methods.

Unlike bulk adaptive immune receptor repertoire profiling, analysis of single T cells or B cells, or few cells in a well reveals paired-chain information of the T-cell receptor (TCR) or B-cell receptor (BCR) and even linked phenotypes, identifying cell subtypes, cell state, and function.

To take advantage of the service, simply send Cellecta FACS-sorted, individual T- or B-cells OR small cell pools of T or B cells, in 96-well plates. Cellecta will run the assay, analyze the results, and send out an analysis report with information on abundant clonotypes, chain pairs, and--in the case of flow-sorted, single T or B cell samples--the highly expressed T- or B-cell markers indicative of specific cell subtypes.

Workflow

Sample Preparation: Sort T- or B-cells (or serially diluted, T-or B-cell pools) in a 96-well plate with TE or PBS buffer, and ship the plate(s) to Cellecta.
DriverMap™ Adaptive Immune Receptor (AIR) Assay & Sequencing: Cellecta will perform the DriverMap AIR multiplex RT-PCR amplification and subsequent sequencing using TCR α/β, TCR γ/δ, or BCR IGH/IGK/IGL primers along with ~30 crucial T- or B-cell markers.
Data Analysis: From the FASTQ files, data is obtained on abundant clonotype sequences, chain pairing information, and highly expressed T- or B-cell markers. A detailed analysis report will be sent to you in a few weeks.

Advantages

Simple: Obtain a highly informative single-cell T-cell or B-cell profile simply and easily. Just send us FACS-sorted T/B cells in a 96-well plate and receive a complete analysis report a few weeks later
Efficient: Avoid the high costs and long timelines associated with the use of complex and expensive microfluidic platforms
Integrated AIR profiling and immunophenotyping data: Acquire essential information on both chain pairing and key phenotype markers (from flow-sorted single T or B cells)

Applications

TCR Gene Rearrangement Analysis: Use paired chain information to clone sequences in Jurkat-NFAT Reporter Cells for epitope prediction studies and potential T-cell-based cancer immunotherapies.
Antibody Development: Utilize full-length IGH/IGK/IGL pairing information for antibody synthesis applications.
Neoantigen Discovery: Gain insights using paired-chain information into antigen-specific TCRs to learn more about their functional significance.

Additional Info

Before using the single-cell profiling service, you may want to consider running a DriverMap AIR bulk repertoire analysis to screen large numbers of samples to find the most abundant clonotypes. This information helps downsample for single-cell analysis to obtain chain pairing information. Cellecta also offers full-suite cloning and packaging capabilities for cDNA constructs or packaged libraries and Jurkat-NFAT Reporter cell lines for antigen screening, neo-antigen, and epitope discovery applications.

Cellecta also offers ready-to-use DriverMap scAIR kits for human, mouse, and hybrid mouse-human samples. With eight 96-well plates included in each kit, this cost-effective and simple assay is ideal for obtaining chain-pair information for a few thousand cells.

Contact info@cellecta.com for more information.

Poster: Single-cell TCRα/β and TCRγ/δ immune receptor profiling and immunophenotyping using a 96-well plate sorted-cell approach

New! Video: A Practical Guide for Immune Receptor Profiling and the Characterization of Antigen-Induced Clonotypes (18:46 min) Alex Chenchik, Cellecta

New! Video: Clonotype-Based Quantitative Profiling of Adaptive Immune Dynamics following Vaccination with TLR5 Agonist (18:31 min) Andrei Gudkov, Roswell Park Cancer Center