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Cloning and Expression Vector Information

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Cellecta offers a comprehensive collection of cloning and expression vectors for individual constructs to pooled library generation. Find all vector information here, including cloning/Next-Gen Sequencing (NGS) cassette and primer design maps, sequences, GenBank/Vector NTI and SnapGene files, and vector maps.

Please note that this list is not comprehensive. Please contact us for information on a specific vector not listed or if interested in a customized vector.

Cellecta’s vector collection includes the following types of expression vectors and accessory vectors. Linearized shRNA/sgRNA vectors or expression vectors with multiple cloning sites are also available, so you may clone your own inserts.

shRNA Knockdown
CRISPR Knockout, CRISPRa Activation, and CRISPRi Repression
CRISPR-Seq (Perturb-Seq)
cDNA Expression
Cell Barcoding

Cellecta can also customize vectors. Please inquire for more information. Choose from a variety of vector elements, and we’ll build your vector:

  • Constitutive or inducible versions of U6 or H1 shRNA/sgRNA expression pol III promoters
  • TagRFP and TagGFP2 fluorescent markers; PuroR, BleoR, NeoR, BlastR, and HygroR antibiotic-resistance markers; mouse Thy1.1 & Thy1.2 cell surface markers; or others of your choice
  • CMV, EF1, EFS, UbiC, PGK, RSV, SFFV, or other pol II promoters for expression of markers


shRNA Knockdown Vectors (Learn more...)






CRISPR, CRISPRa, and CRISPRi sgRNA Expression Vectors


Single-Vector CRISPR sgRNA/Cas9 Knockout System (Learn more...)


sgRNA-Only Expression Vectors (Two Vector System) (Learn more...)





Dual-sgRNA Expression Vectors (Learn more...)





Cas9- and dCas9-Only Expression Vectors

Cas9 Vectors for CRISPR Knockout (Learn more...)


dCas9-VPH Vectors for CRISPRa Activation (Learn more...)


dCas9-KRAB Vectors for CRISPRi Repression (Learn more...)



CloneTracker XP™ Expressed sgRNA-Barcode and sgRNA-Only Vectors (Learn more...)




cDNA Expression Vectors (Learn more...)




InDOXible™ Dox-Activated cDNA Expression System (Learn more...)




Cell Barcoding Vectors

CloneTracker XP™ Expressed Barcode Vectors (Learn more...)



* Only vectors with NGS cassettes can be used for library construction.

** Sequences are available in FASTA (.txt), GenBank (.gb/.gbk), and SnapGene (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us.



Lentiviral Vector Safety Information

For information on the safety of our lentiviral vectors, please download our Safety Data Sheets (SDS):