Catalog #: CVCRC

Custom sgRNA Constructs

Knockout, knockdown, or activation your target gene with CRISPR, CRISPRi, or CRISPRa sgRNA lentiviral constructs.

  • Flexible design services to ensure the sgRNA target the genes, isoforms, or regulatory elements you want
  • Get lentiviral plasmids and pre-packaged lentiviral particles
  • A range of vector designs, including sgRNA-only, sgRNA-Cas9, dual-sgRNA, inducible sgRNA vectors
  • Non-targeting, non-specific, and other control guides avalable in any vector backbone

We will design, make, and deliver whatever CRISPR sgRNA constructs you need.

Custom sgRNA ConstructsThe CRISPR/Cas9 system can be used for gene knockout (KO), knockdown, activation or to initiate knock-ins in vivo or in vitro by using a combination of an sgRNA (single-guide RNA) together with

a Cas9 nuclease. Some examples of custom constructs include....

  • Constitutive or tet-inducible sgRNA constructs designed for CRISPR KO, CRISPRa, or CRISPRi.
  • All-in-one constructs with sgRNA and Cas9 or single-vector sgRNA-only formats.
  • Complete panels of Cas9 and dCas9-hybrid (e.g., dCas9-KRAB, dCas9-VPH, dCas9-VPR, etc.) expression constructs

Cellecta offers both two-vector (i.e., sgRNA-only constructs with Cas9 or dCas9 variants on a separate construct) and single-vector sgRNA-Cas9 constructs (i.e., one construct expressing both the Cas9 gene and your sgRNA).

CRISPR sgRNA constructs targeting GFP were built using the Two Vector and Single Vector CRISPR systems and used to transduce cells that had been previously engineered to stably express GFP. Both CRISPR systems provide at least 80% knockout of GFP, but the two-vector system performed better with over 90% knockout. The enhanced performance is due to pre-selection of cells expressing high levels of Cas9 before transduction with the GFP sgRNA constructs.

Just provide the gene information and we will select the optimal guide designs.

Get the CRISPR KO, CRISPRi, or CRISPRa sgRNA constructs you want, simply and easily.

Not finding what you are looking for? We are here to help.

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