Functional Genetic Screens with Pooled CRISPR / shRNA Libraries 

Uncover gene function, drug targets, and mechanisms of action with genome-wide or custom pooled libraries and end-to-end screening support.

Overview

Cellecta’s pooled CRISPR and shRNA platforms enable high-throughput screening to identify genes linked to diverse cellular phenotypes— viability, drug resistance, pathway activation, synthetic lethality, and more.

Whether you want to run your own screen or fully outsource the work, Cellecta offers both ready-to-use libraries and start-to-finish functional screening services. Our team can manage the entire workflow—from engineering Cas9-expressing cells to final analysis—freeing your lab to focus on discovery.

Applications

• Genome-wide or pathway-focused gene knockout and knockdown screens
• Drug resistance and sensitization discovery
• Synthetic lethal interaction mapping
• Pathway and reporter-based enrichment screens
• Tumorigenesis and metastasis screens in vivo

Functional Screening Workflow

• Cell Line Engineering – Cas9 delivery, inducible systems, or reporter constructs
• Library Delivery – Transduction or transfection of sgRNA or shRNA libraries
• Selection and Treatment – Drug exposure, phenotypic selection, or enrichment protocol
• Sample Collection – DNA/RNA extraction and barcode amplification
• Next-Generation Sequencing (NGS) + Data Analysis – Guide quantification, hit calling, downstream bioinformatics analysis, result interpretation

Our Expertise and Differentiators

• Almost Two Decades of Screening Experience
• Both CRISPR and RNAi Platforms
• HEAT-Modified CRISPR Guides
• Ready-to-Use or Custom Libraries
• End-to-End Screening Services
• Validated Performance Across Models
• Support for In-Lab Screening

Start-to-Finish Screening Services

• Cell line engineering (e.g., Cas9 integration)
• Library transduction or transfection
• Culture management and experimental treatment
• DNA/RNA extraction and barcode amplification
• NGS and enrichment analysis

Get Started

1. CRISPR/RNAi Screening Services
2. Single-Cell CRISPR Screening Service
3. Custom Cas9 and dCas9 Variant Cell Lines
4. Custom Engineered Cell Lines
5. Sequencing Service for Samples from Genetic Screens
6. Bioinformatics Analysis Services