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Pooled sgRNA Library targeting all human protein-coding genes and designed to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression. Five sgRNA to each gene target. Library includes 3,700 non-targeting sgRNA controls. Designs based on Horlbeck, et al., eLife 2016;5:e19760 DOI: 10.7554).
* Sequences are available in FASTA (.txt), GenBank (.gb), and SnapGene (.dna) formats. You may use thefree SnapGene Viewerto open .dna files. If you would like vector files in another format, pleasecontact us. To download .txt and .gb files, right click on the link and Save As.