DriverMap™ AIR TCR-BCR Profiling Kit (Human RNA)

$1,650.00

U.S. List Price more info: Prices shown reflect U.S. list pricing for orders shipped to U.S. addresses. Non-U.S. pricing may vary depending on geography. Please contact orders@cellecta.com for a quote or check our distributor page to see if Cellecta has an authorized reseller in your region.

Product Information

Easy-to-run, single-day assay that uses multiplex PCR-NGS technology to profile T-cell receptor (TCR) and / or B-cell receptor (BCR) repertoire starting from total RNA.

Reagent kits are available for full-length or only CDR3 region profiling of all 4 TCR chains (TRA, TRB, TRG, and TRD) AND 3 BCR chains (IGH, IGK, and IGL) in a single reaction from a single sample.
Reagent kits for full-length or CDR3 profiling of either TCR (4 chains) orBCR (3 chains) are also available.
Optimized RT-PCR workflow. No specialized equipment required.
Run in parallel with theDriverMap AIR TCR-BCR (Human DNA) kit with DNA starting material for insights into activated clonotypes
Pre-set data analysis pipelinesusing MiXCR (MiLaboratories) immune receptor profiling software; compatible with other similar analysis packages.

Comparison of CDR3 vs Full-Length Variable Region Repertoire Profiling

The table below summarizes the key differences between profiling only the CDR3 region and profiling the full-length variable region.

	DriverMap AIR TCR-BCR (CDR3 only) Profiling	DriverMap AIR TCR-BCR (Full-Length Variable Region) Profiling
Region	CDR3 only	FR1, FR2, FR3, CDR1, CDR2 and CDR3
Amplicon Length (bp)	~ 300 bp	~ 600 bp
Primer Location
Starting Material	RNA or DNA	RNA
Model System	Human (RNA or DNA)/ Mouse (RNA)	Human
NGS Instrument Options	Illumina NextSeq500/550, NextSeq 2000, NovaSeq	Illumina NextSeq 2000 (x 2 300 cycles) kit only
How it Works	The CDR3 region determines the structure and specificity of the antigen. The majority of repertoire profiling studies are based on analysis of only the CDR3 region, as it is the hypervariable region and contains crucial information for studying immune repertoire diversity.	The V region encodes CDR1 and CDR2 in germline DNA segments and primarily interacts with the major histocompatibility complex (MHC) molecules. The full-length receptor region is involved in antigen receptor binding affinity and/or downstream signaling and allows one to clone and express the identified and chosen receptors.
Key Applications	Tracking T cell diversity and clonality Finding markers of autoimmune diseases Studying immune cell migration patterns	Antibody and vaccine development T-cell epitope discovery

Workflow Requirements

Each DriverMap™ AIR TCR-BCR Profiling Reagent Kit includes all specialized enzymes, primers, and reagents required for target enrichment and library preparation; however, to run the libraries on Illumina® next-generation sequencing (NGS) platforms, a compatible Universal Unique Dual Index (UDI) NGS Index Plate must be purchased separately as a required component of the workflow.

Purchase both

1) DriverMap AIR Reagent Kit AND 2) Universal Unique Dual Index (UDI) NGS Plate

Catalog #	DriverMap AIR Reagent Kit	Quantity
DMAIR2-HBR-24-B1	DriverMap™ AIR RNA CDR3 BCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HBR-96-B1	DriverMap™ AIR RNA CDR3 BCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >
DMAIR2-HTBR-24-B1	DriverMap™ AIR RNA CDR3 TCR-BCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HTBR-96-B1	DriverMap™ AIR RNA CDR3 TCR-BCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >
DMAIR2-HTR-24-B1	DriverMap™ AIR RNA CDR3 TCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HTR-96-B1	DriverMap™ AIR RNA CDR3 TCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >
DMAIR2-HTBR-F-24-B1	DriverMap™ AIR RNA Full-length TCR-BCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HTBR-F-96-B1	DriverMap™ AIR RNA Full-length TCR-BCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >
DMAIR2-HTR-F-24-B1	DriverMap™ AIR RNA Full-length TCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HTR-F-96-B1	DriverMap™ AIR RNA Full-length TCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >
DMAIR2-HBR-F-24-B1	DriverMap™ AIR RNA Full-length BCR Profiling Reagent Kit (Human), V2	1 kit (for 24 reactions)	Order Now >
DMAIR2-HBR-F-96-B1	DriverMap™ AIR RNA Full-length BCR Profiling Reagent Kit (Human), V2	1 kit (for 96 reactions)	Order Now >

Required Product

Catalog #	Universal Unique Dual Index (UDI) NGS Plate	Quantity
DM-NGS-SetA-B2	Unique Dual Index Set A for use withDriverMap EXP or AIR Reagent Kit (1-96)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetB-B2	Unique Dual Index Set B for use with DriverMap EXP or AIR Reagent Kit (97-192)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetC-B2	Unique Dual Index Set C for use with DriverMap EXP or AIR Reagent Kit (193-288)	1x 96-Well Plate (4 rxn/well)	Order Now >
DM-NGS-SetD-B2	Unique Dual Index Set D for use with DriverMap EXP or AIR Reagent Kit (289-384)	1x 96-Well Plate (4 rxn/well)	Order Now >

Manual: DriverMap AIR RNA Profiling Kit Manual - for the AIR RNA kits profiling the CDR3 regions.
Manual: DriverMap AIR TCR/BCR Full-Length Profiling Kit Manual - for the AIR RNA profiling of the full-length region

Technology Guide: DriverMap AIR Technology Guide for an introduction to AIR technology and for recommendations on designing an AIR profiling experiment

Product Flyers and other Resources:
Tech Notes / Blogs
- Comparing DNA vs. RNA Samples for Immune Repertoire Profiling

DriverMap™ AIR TCR-BCR Profiling Kit (Human RNA)g - Kits

Fig 1: Number of clonotypes for 7 TCR/BCR chains identified in 50ng of normal PBMC or whole blood RNA (10x10^6 reads per sample, triplicates).

Fig 2: Comparison of TCR clonotypes detected by DriverMap AIR RNA full-length assay vs SMART-based assay. Both assays were run with 50 ng total RNA isolated from PBMCs. Data shows that the DriverMap AIR RNA assay detects approximately 3X more TCR clonotypes than the SMART-based assay.