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Easy-to-run, single-day assay that uses multiplex PCR-NGS technology to profile T-cell receptor (TCR) or B-cell receptor (BCR) repertoire present in human DNA. The DriverMap AIR DNA assay measures the frequency of CDR3 clonotypes to quantify the clonal frequency of T and B cells.
Assay primers contain unique molecular identifiers (UMIs) to enable accurate quantitation of each clonotype.
Sensitive profiling of samples with low lymphocyte content (e.g. FFPE, tumor biopsy, achieved specimens) where DNA is more stable than RNA.
Complete kit includes all the enzymes, reagents, and primers to prepare 24- or 96- samples ready for multiplex Illumina NGS runs from DNA starting material. No additional specialized equipment required.
Parallel runs of DriverMap AIR DNA Assay with DriverMap AIR RNA Assay reveal potential disease-associated, activated CDR3 clonotypes
Fig 1: Mean clonotype counts for four TCR chains from different DNA sample inputs.