DriverMap™ AIR TCR-BCR Profiling Kit (Human RNA)

$1,700.00

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Product Information

Easy-to-run, single-day assay that uses multiplex PCR-NGS technology to profile T-cell receptor (TCR) and / or B-cell receptor (BCR) repertoire starting from total RNA.

Profile all 4 TCR chains (TRA, TRB, TRG and TRD) and 3 BCR chains (IGH, IGK and IGL) in a single reaction from a single sample.
Assay kits for profiling of only TCR (4 chains) or BCR (3 chains) also available.
Includes all the enzymes, reagents, and primers to run single-tube, multiplex assays that generate NGS-ready libraries for 24- or 96- samples.
Optimized RT-PCR workflow. No specialized equipment required.
Run in parallel with the DriverMap AIR TCR-BCR (Human DNA) kit with DNA starting material for insights into activated clonotypes
Data compatible with analysis using MiXCR (MiLaboratories) immune receptor profiling software and similar analysis packages.

DriverMap™ AIR TCR-BCR Profiling Kit (Human RNA)g - Kits

Fig 1: Number of clonotypes for 7 TCR/BCR chains identified in 50ng of normal PBMC or whole blood RNA (10x10^6 reads per sample, triplicates).

Fig 2: Comparison of TCR clonotypes detected by DriverMap AIR RNA full-length assay vs SMART-based assay. Both assays were run with 50 ng total RNA isolated from PBMCs. Data shows that the DriverMap AIR RNA assay detects approximately 3X more TCR clonotypes than the SMART-based assay.

Comparison of CDR3 vs Full-Length Variable Region Repertoire Profiling

The table below summarizes the key differences between profiling only the CDR3 region vs profiling of the full-length variable region.

	DriverMap AIR TCR-BCR (CDR3 only) Profiling	DriverMap AIR TCR-BCR (Full-Length Variable Region) Profiling
Region	CDR3 only	FR1, FR2, FR3, CDR1, CDR2 and CDR3
Amplicon Length (bp)	~ 300 bp	~ 600 bp
Primer Location
Starting Material	RNA or DNA	RNA
Model System	Human (RNA or DNA)/ Mouse (RNA)	Human
NGS Instrument Options	Illumina NextSeq500/550, NextSeq 2000, NovaSeq	Illumina NextSeq 2000 (x 2 300 cycles) kit only
How it Works	The CDR3 region determines the structure and specificity of the antigen. The majority of repertoire profiling studies are based on the analysis of only the CDR3 region, as this is the hypervariable region and contains crucial information to study immune repertoire diversity.	The V region encodes CDR1 and CDR2 in germline DNA segments and primarily interacts with the major histocompatibility complex (MHC) molecules. The full-length receptor region is involved in antigen receptor binding affinity and/or downstream signaling and allows one to clone and express the identified and chosen receptors.
Key Applications	Tracking T cell diversity and clonality Finding markers of autoimmune diseases Studying immune cell migration patterns	Antibody and vaccine development T-cell epitope discovery

Manual: DriverMap AIR RNA Profiling Kit Manual - for the AIR RNA kits profiling the CDR3 regions.
Manual: DriverMap AIR TCR/BCR Full-Length Profiling Kit Manual - for the AIR RNA profiling of the full-length region

Technology Guide: DriverMap AIR Technology Guide for an introduction to AIR technology and for recommendations on designing an AIR profiling experiment