DriverMap Single-Cell AIR TCR-BCR Assay Kits

Obtain full-length, paired receptor sequences from flow-sorted or diluted T or B cells with the DriverMap Single-Cell Adaptive Immune Receptor (scAIR) TCR-BCR assays. These consist of a variety of easy-to-use, 96-well, plate-based RT-PCR kit configurations providing:

• Full-length, chain-pair receptor sequences: Obtain complete coding region sequences for human, mouse, and mouse/human hybrid T-cell (TRA-TRB) or B-cell (IGH-IGK-IGL) receptor chain pairs.
• Simple, streamlined protocol: Sort cells into an indexed 96-well plate with RT primers, hybridize, then pool samples into a single reaction tube. Perform RT-PCR and next-generation sequencing (NGS), scTCR-Seq or scBCR-Seq. No RNA extraction, specialized equipment, or complex barcoding required.
• Identify TRA/TRB or IGH/IGK/IGL chain pairs from single cells or 100s of cells per well: Two workflow options--the first identifies TCR or BCR chain pairs from flow-sorted individual cells; the second starts from populations of up to 200 cells per well to maximize chain-pairing information from the most abundant receptors and enable cost-effective, efficient scaling of experiments
• Flexible and cost-effective: Economical analysis, starting from approximately 10 cents per cell, and optional immune cell marker profiling data obtained from the same sample run
• Integrated bioinformatics analysis pipeline with MiXCR software on Platforma (MiLaboratories, Inc.) to process FASTQ files, obtain clonotype alignment and chain-pair information, and optionally, the immunophenotype with information on cell function and state. Other open-source tools may also be used for further data processing.

TheDriverMap ™scAIRTCR/BCRKits include all the enzymes, reagents, and primers to preparesamples for profilingusing next-generation sequencing (NGS), scTCR-Seq or scBCR-Seq.

Fig 1: Number of TCR α/β chain pairs identified in a 96-well plate with the DriverMap scAIR TCR assay in human PBMC sample at 50, 100, and 200 cells per well (left) and Number of clonotypes with matchedα/β chains per plate (right). Results show consistent and reproducible TCR chain pairing across the three tested cell-input levels.

Fig 2: DriverMap scAIR TCR-BCR assay workflow. The assay workflow highlights the simplicity of the protocol, starting from flow-sorted cells through the PCR-based immune profiling and optional immunophenotyping to NGS and data analysis steps.

 

DM-SCAIR-HTR	DriverMap™ scAIR TCR Chain Profiling Kit (Human)	1 kit (for #8-96-well plates)
DM-SCAIR-MTR	DriverMap™scAIR TCR Chain Profiling Kit (Mouse)	1 kit (for #8-96-well plates)
DM-SCAIR-HBR	DriverMap™scAIR BCR Chain Profiling Kit (Human)	1 kit (for #8-96-well plates)
DM-SCAIR-MBR	DriverMap™scAIR BCR Chain Profiling Kit (Mouse)	1 kit (for #8-96-well plates)
DM-SCAIR-HTR-M36	DriverMap™scAIR TCR-Mark36 Immunophenotyping Kit (Human)	1 kit (for #8-96-well plates)
DM-SCAIR-HBR-M30	DriverMap™scAIR BCR-Mark30 Immunophenotyping Kit (Human)	1 kit (for #8-96-well plates)
DM-SCAIR-HM-TR	DriverMap™scAIR TCR Chain Profiling Kit (Hybrid Human-Mouse)	1 kit (for #8-96-well plates)
DM-SCAIR-HM-BR	DriverMap™scAIR BCR Chain Profiling Kit (Hybrid Human-Mouse)	1 kit (for #896-well plates)

The DriverMap scAIR assay is also available as a service, where you would send us your flow-sorted cells on a 96-well plate, and Cellecta will send back a full analysis report. More information on the service here >

For more information on clonal repertoire diversity and more, you may want to consider the DriverMap AIR assay for bulk samples. More information on the DriverMap bulk AIR assay is available here >

Manual and Product Resources

• Manual: DriverMap™ Single-cell AIR TCR-BCR Chain Pairing Assay (Human, Mouse, Hybrid Human-Mouse) - V2 

• Manual: DriverMap™ Single-cell AIR TCR-BCR Immunophenotyping Assay (Human) - V2 

• Technology Guide: DriverMap AIR Technology Guide for an introduction to AIR technology and for recommendations on designing an AIR profiling experiment

• Product Flyers and other Resources:

  • Flyer: Comprehensive Adaptive Immune Receptor Profiling for All Immune Sample Types

  • Flyer : Synthetic Spike-in Controls for Immune Repertoire Profiling

  • Brochure : DriverMap Technology Solution

  • Poster: A novel 96-well plate-based FACS-sorted single-cell approach allows the identification of immune receptor chain pairing & immunophenotyping of T or B cells

  • Video: Immunophenotyping of T and B-Cell Receptors; by Alex Chenchik, Cellecta, Inc

• Tech Notes / Blogs

• Comparing DNA vs. RNA Samples for Immune Repertoire Profiling