DriverMap™ EXP Human Genome-Wide Expression Profiling Kit, V3

The DriverMap™ Targeted RNA Sequencing Expression Profiling (EXP) Assay is a convenient single-tube RT-PCR/Next-Generation Sequencing (NGS) assay that robustly measures expression levels of ~19,000 human protein-coding genes from ng levels of total RNA in blood, cells, tissues, and even FFPE samples. We have designed primers 80-200 nt in length to target the conserved regions of all human protein-coding genes and optimized them for reliable results in an efficient, robust multiplex assay.

If you want a quick snapshot of up-regulated and down-regulated genes, even from small or rare samples, and do not require information on alternative splice variants, this assay is worth considering.

• DriverMap Targeted RNA-Seq EXP 19K Human Genome-Wide Kits are available in two formats, depending on the samples run:

1. 1. DriverMap EXP Human Genome-Wide 19K Profiling Reagent Kit  (starting material: blood, tissue, or cells) with all the enzymes, primers, and reagents required for target enrichment and NGS library preparation.
   2. DriverMap EXP Human Genome-Wide 19K Dried Blood Microsample Profiling Reagent Kit (starting material: low amounts of blood). In addition to all the reagents in the kit above, the dried blood microsample kit contains additional reagents for RNA extraction from the Mitra blood microsampler tips.
      

• Open-source Salmon software is used to quantify gene expression levels. Further downstream analysis may be carried out using a variety of differential gene expression software tools.

• DriverMap Targeted RNA-Seq Expression (EXP) Profiling is also available as a custom service. 

Workflow Requirements 

Each DriverMap™ EXP 19K Genome-Wide Profiling Reagent Kit includes all specialized enzymes, primers, and reagents required for target enrichment and library preparation; however, to run the libraries on Illumina® next-generation sequencing (NGS) platforms, a compatible Universal Unique Dual Index (UDI) NGS Index Plate must be purchased separately as a required component of the workflow.

• DriverMap EXP Human Genome-Wide 19K Profiling Reagent Kit  (starting material: blood, tissue, or cells, 24- or 96-sample kits) 

• Required Product for Illumina NGS Run (Must purchase in addition to Reagent Kit)

• DriverMap EXP Human Genome-Wide 19K Dried Blood Microsample Profiling Reagent Kit (low amounts of blood, 24- or 96-sample kits) 
  
  • This innovative solution for transcriptomic analysis of small volumes of whole blood (e.g. 30 μl) is a convenient and reliable way of obtaining high-throughput gene expression data for monitoring gene regulation and expression for biomarker and drug discovery applications. With this method, easy sample collection from remote locations without access to traditional blood collection facilities is possible.
  • The DriverMap EXP Dried Blood kit contains additional reagents for RNA extraction from the Mitra tips, as well as all the other primers and reagents required for NGS library prep included in the original DriverMap EXP Profiling kit.
    • A Cellecta Universal Unique Dual-Index (UDI) NGS Plate must be purchased to complete the NGS step of the workflow.
  • Finally, to use the DriverMap EXP Dried Blood protocol, you will need to order the Neoteryx Mitra tips and GenTegra GTR-NEO Active Chemical Protection of RNA solution separately from the Neoteryx site according to the table below:

The figure above shows reproducible, sensitive expression profiling with >90% overlap in gene expression profiles obtained from two different donors, whose blood was extracted using standard blood collection tubes (400 ul) and Mitra tips (30 ul), with a strong correlation (r=0.94) between the results.

Manual and Product Documentation

• Manual: DriverMap Targeted Expression Profiling Kits (for blood, cells, tissue)
• Manual: DriverMap Targeted Expression (EXP) Dried Blood Microsampling Profiling Kits (for dried blood samples obtained using the Neoteryx Mitra device)
• Brochure: DriverMap™ Human Genome-Wide Expression Profiling Solution
• Reference: Publication: Tarca et al., Scientific Reports, (2019) 9:848 Targeted expression profling by RNA-Seq improves detection of cellular dynamics during pregnancy and identifes a role for T cells in term parturition
• Application Note: Convenience and Power: Whole Blood Transcriptome Profiling Using Microsampling 
• Microsampling Poster: Microsampling for RNA Biomarker Profiling in Blood
• Gene list: Gene List DriverMap Human Genome-Wide v3

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