Single-Cell DriverMap AIR T-Cell and B-Cell Receptor Profiling Service
Cellecta offers a simple and efficient single-cell adaptive immune receptor (AIR) profiling service starting from FACS-sorted T- and B-cells in a 96-well plate format, avoiding the workflow complexity and high cost of microfluidics-based, single-cell workflows.
Unlike bulk adaptive immune receptor repertoire profiling, single-cell analysis of the T-cell receptor (TCR) or B-cell receptor (BCR) reveals paired-chain information and linked phenotypes, such as cell subtypes.
To take advantage of the service, simply send Cellecta FACS-sorted, individual T- or B-cells in 96-well plates. Cellecta will run the assay, analyze the results, and send out an analysis report with information on abundant clonotypes, chain pairing, and highly expressed T- or B-cell markers.
Workflow
Sample Preparation: Sort T- or B-cells in a 96-well plate with TE or PBS buffer, and ship the plate(s) to Cellecta.
DriverMap™ Adaptive Immune Receptor (AIR) Assay & Sequencing: Cellecta will perform the DriverMap AIR multiplex RT-PCR amplification and subsequent sequencing using TCR α/β, TCR γ/δ, or BCR IGH/IGK/IGL primers along with 30 crucial T- or B-cell markers.
Data Analysis: From the FASTQ files, data is obtained on abundant clonotype sequences, chain pairing information, and highly expressed T- or B-cell markers. A detailed analysis report will be sent to you in a few weeks.
Advantages
Simple: Obtain a highly informative single-cell T-cell or B-cell profile simply and easily. Just send us FACS-sorted T/B cells in a 96-well plate and receive a complete analysis report a few weeks later
Efficient: Avoid the high costs and long timelines associated with the use of complex and expensive microfluidic platforms
Integrated AIR profiling and immunophenotyping data: Acquire essential information on both chain pairing and key phenotype markers
Applications
TCR Gene Rearrangement Analysis: Use paired chain information to clone sequences in Jurkat-NFAT Reporter Cells for epitope prediction studies and potential T-cell-based cancer immunotherapies.
Antibody Development: Utilize full-length IGH/IGK/IGL pairing information for antibody synthesis applications.
Neoantigen Discovery: Gain insights using paired-chain information into antigen-specific TCRs to learn more about their functional significance.
Additional Services
Combine the single-cell profiling service with DriverMap AIR bulk repertoire analysis to screen a large number of samples to find the most abundant clonotypes. This information helps downsample for single-cell analysis to obtain chain pairing information. Cellecta also offers full-suite cloning and packaging capabilities for cDNA constructs or packaged libraries and Jurkat-NFAT Reporter cell lines for antigen screening applications.
