Catalog #: CPLVBCL
Limited complexity–a small number of defined barcodes. Our off-the-shelf libraries with 1M, 10M, and 50M barcodes may be too complex for your experimental needs. Some studies looking at population distributions may only require a few thousand barcodes. We can certainly provide smaller libraries with 1000, 5000 or 20,000 unique, distinct barcodes. With these libraries of limited complexity, deeper sequencing of the whole library is possible.
Specialized markers or selection. For some barcode tracking experiments, a different marker or selection may be required, such as Thy1.1, luciferase, or thymidine kinase. We can readily generate a barcode library of any size in a specialized vector for you.
Barcoding combined with other effectors or elements in a library. As deep sequencing has become much more accessible, barcode tracking has become a useful tool for tracking cell-to-cell variations in conjunction with a variety of assays. For example, several groups have focused on using barcode tracking to assay RNA expression in conjunction with pooled CRISPR screening (aka., Perturb-Seq, CRISPR-Seq, CROP-Seq). Cellecta is able to provide libraries that combine barcodes with sgRNA, shRNA, etc. and offers the pScribe Vectors so that the barcodes are transcribed and trackable by RNA sequencing, as well as genomic DNA analysis.