Cellecta Inc. offers comprehensive T- and B-cell receptor sequencing (TCR-Seq or BCR-Seq) and analysis, focusing on the full-length or CDR3 receptor region. These adaptive immune receptor repertoire (AIRR) profiling analysis services include clonotype alignment, data processing, visualization, and post-analysis using MiLaboratories MiXCR software pipeline. This service is available to Cellecta’s DriverMap AIR profiling customers and others using the multiplex PCR- and SMART technology-based bulk profiling assays mentioned here.
The standard MiXCR alignment and repertoire analysis report includes assessment of sequencing and alignment quality, analysis of TCR and BCR chains, and visualization of clonotypes, diversity metrics, and gene usage information.
Fig. 1: Overview of Cellecta TCR-Seq and BCR-Seq Bioinformatics Analysis Service
Our advanced analysis report offers additional in-depth insights, including differential clonotype analysis, clonotype activation analysis, and antigen detection.
Fig. 2: Compare TCR or BCR clonotypes from different samples to identify similarities or differences in the immune response.
Fig. 3: Clonotype activation analysis illustrating differences in IGH clonotype levels in DNA and RNA pre- and post-vaccination.
Fig. 4: Lineage tree mapping for full-length TCR and BCR analysis allows visualization of evolutionary relationships between different immune cell clones.
Fig. 5: Clone tracking identifies and monitors specific T or B cell clones in an immune repertoire over time or across conditions.
Fig. 6: ImmuneWatch DETECT software predicts epitopes matching the TCR-activated clonotypes identified by the DriverMap AIR Profiling Assay.
We also offer custom analysis based on your specific research needs.
Contact us at info@cellecta.com to discuss your specific immune receptor profiling and bioinformatics analysis requirements. Our expert team is here to work with you to support your research.
