DriverMap™ DirectCell™ Gene Expression Profiling Protocol

All the benefits of the DriverMap Targeted Expression Profiling assay together with a simple, plate-based RNA isolation protocol

• Incubate cell lysate in poly-A+ wells, wash, run DriverMap RT-PCR assay for profiling
• No transfers or additional purification steps
• Ultra-sensitive expression profiling, even from individual cells

The DriverMap Targeted Expression Profiling assay, a PCR-based approach, yields reliable data even from very low amounts of RNA. When used with mRNA retrieved by hybridization on an oligo-dT plate, simply wash and multiplex RT-PCR in the same wells. No additional transfers, precipitation, or centrifuging are required. The samples are transferred into a second index plate to prepare sequence-ready libraries.

The DriverMap DirectCell protocol is simple

1. Introduce cells into oligo-DT plate
2. Run your cell assay
3. Lyse cells and rinse
4. Add DriverMap gene-specific RT primer mix
5. Run DriverMap assay to produce sequence-ready library
6. Sequence

More information on the DriverMap assay and workflow is available here.