Gene Knockdown or Knockout Validation

Cellecta can help validate target genes by knocking down expression in your cells of interest. Cellecta provides complete services for validation of target genes including qRT-PCR, proliferation assays, reporter assays, and custom stable knockdown or knockout cell line creation.

Validation of Target Gene Knockdown or Knockout

We design and make several constructs to the target gene, then test these to find the one with the best knockdown or knockout:

• You tell us the RefSeq number or gene ID of the target gene you want to knock down or knock out. We do the rest.
• Best knockdown is measured by qRT-PCR, and is usually better than 70%
• Knockout is measured by sequencing of both alleles
• We can provide you with the confirmed shRNA or sgRNA construct for the gene target
• Alternatively, we can send you the stable pool we used for testing
• 10 week turnaround time

Proliferation, Survival, or Viability Assays

• Quantify growth rates, survival, or cell death by FACS analysis
• Uses tet-inducible shRNA or sgRNA expression to compare growth rate of induced vs. uninduced cells

Individual shRNA or sgRNA Validation in Inducible Vector

• Quantify growth rates, survival, or cell death by FACS analysis
• Uses tet-inducible shRNA or sgRNA expression to compare growth rate of induced vs. uninduced cells

The price for this type of screen typically depends on the treatment variations, number of cell lines, types of cells, and related specifics. If you are interested in having Cellecta validate knockdown or knockout, please contact us at sales@cellecta.com.