European Association for Cancer Research 2022 Congress

June 20-23, 2022, Seville Spain, FIBES - Seville Exhibition and Conference Center

A.   Industry Symposium:

Wednesday, June 22 at 08:00-08:45 h, Room Paris + Brussels

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Title:  Single Cell CRISPR Screening Identifies Non-Coding RNA Modulating Cancer Plasticity

Moderator: Paul Diehl, PhD, Chief Operating Officer, Cellecta

Speakers:

Donato Tedesco, PhD, Director of Research & Development, Cellecta

Perturb-seq analysis of TNFalpha-induced transcriptional response

Key points:

• We identified genes involved in TNFalpha-induced transcriptional response using two methods: a) Perturb-Seq screen and an b) arrayed screen
• We determined the minimum number of cells per sgRNA needed to cover the full complexity of the pooled library for the Perturb-Seq experiment
• Analysis was carried out using a transcription profiling assay for the arrayed screen vs CellRanger software for the Perturb-Seq screen. Both methods yielded comparable results.

Matteo Marzi, PhD, Istituto Italiano di Tecnologia

A functional map of LncRNAs involved in adaptation pathways of breast cancer cells

Key points:

• We identified lncRNAs associated with breast cancer transcriptional plasticity
• We will share a design of an effective CROP-seq sgRNA library for targeting lncRNAs by CRISPRi
• We will present a functional map of lncRNA loci involved in cancer plasticity leveraging multiple competitive sgRNA screens

Register to attend in person

I won't be able to attend in person but am interested in receiving the recording of the talks

B.   Industry Spotlight:

Wednesday, June 22 from 16:45-17:00 h, Spotlight Area

Speaker: Paul Diehl, PhD, Chief Operating Officer, Cellecta, Inc.