European Association for Cancer Research 2022 Congress

June 20-23, 2022, Seville Spain, FIBES - Seville Exhibition and Conference Center

Click here for event program

A.   Poster Presentations

     1. Donato Tedesco, PhD

    • Title: Key mediators of TNF-alpha transcriptional response identified by arrayed and pooled sgRNA library Perturb-seq screen
    • Poster Session 2, June 22 from 10:15-18:00 
     2. Nadya Isachenko, PhD
    • Title: Genome editing for stem cells
    • Poster Session 2, June 22 from 10:15-18:00

     3. Paul Diehl, PhD

    • Title: Ultra-sensitive immunophenotyping of TCR and BCR clonotypes
    • Poster Session 2, June 22 from 10:15-18:00

 

B.   Industry Symposium:

Wednesday, June 22 at 08:00-08:45 h, Room Paris + Brussels

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Title:  Single Cell CRISPR Screening Identifies Non-Coding RNA Modulating Cancer Plasticity

Moderator: Paul Diehl, PhD- Chief Operating Officer, Cellecta, Inc.

Speakers:

Donato Tedesco, PhD– Cellecta, Inc.

Perturb-seq analysis of TNFalpha-induced transcriptional response

Key points:

  • We identified genes involved in TNFalpha-induced transcriptional response using two methods: a) Perturb-Seq screen and an b) arrayed screen
  • We determined the minimum number of cells per sgRNA needed to cover the full complexity of the pooled library for the Perturb-Seq experiment
  • Analysis was carried out using a transcription profiling assay for the arrayed screen vs CellRanger software for the Perturb-Seq screen. Both methods yielded comparable results.

Matteo Marzi, PhD – Istituto Italiano di Tecnologia

A functional map of LncRNAs involved in adaptation pathways of breast cancer cells

Key points:

  • We identified lncRNAs associated with breast cancer transcriptional plasticity
  • We will share a design of an effective CROP-seq sgRNA library for targeting lncRNAs by CRISPRi
  • We will present a functional map of lncRNA loci involved in cancer plasticity leveraging multiple competitive sgRNA screens

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I won't be able to attend in person but am interested in receiving the recording of the talks

 

C.   Industry Spotlight:

Wednesday, June 22 from 17:00-17:15 h, Exhibit Hall

Speaker: Paul Diehl, PhD, Chief Operating Officer, Cellecta, Inc.

Overview of Cellecta