Thy1.1 Gene as Marker in Cellecta Custom Constructs

Cellecta now offers the mouse Thy1.1 gene (Thymus Cell Antigen 1.1, CD90.1) as a marker in custom CRISPR, RNAi, and cDNA constructs.

Thy1 genes express small, almost peptide-like T-cell glycooprotein expressed by thymocytes, hematopoietic stem cells, some fibroblast and muscle cells in most mammals. These glycoproteins anchor to the cell membrane and seem to play a role in cell-cell interactions.

Mice happen to have two distinct alleles of this gene—Thy1.1 and Thy1.2—that differ by only one amino acid, and commercially available antibodies are available that recognize each of these Thy1 variants specifically. Of the two Thy1 variations, Thy1.1 is only found in a limited number of mouse strains (e.g., B6 Thy1.1 mouse strain).

Since Thy1.1 is limited to a narrow range of hematopoietic cells in just a few mouse strains and mice do not respond immunogenically to it, the Thy1.1 gene can be used as and readily detectable cell marker for in vivo mouse studies. Cells expressing this gene can be conveniently identified and even separated by FACS, IHC, and other antibody-based techniques. For example, studies have used this marker to track engineered cells implanted in mice, and as a baseline across sub-populations of implanted cells expressing different reporters, such as two implanted populations of cells with each expressing a different luciferase variant.

To make this useful marker more accessible to researchers, Cellecta now offers the option to substitute Thy1.1 for either the fluorescent marker or antibiotic selection gene in any of our standard sgRNA, shRNA, or cDNA lentiviral vectors.

Flow Cytometry of cells transduced with RFP and Thy1.1 gene
Four populations of 293T cells were analyzed by fluorescent-activated flow cytometry: an unmodified population, cells transduced with a lentiviral construct with the red fluorescent protein (RFP) gene, cells transduced with a lentiviral construct with the Thy1.1 gene, and cells transduced with a lentiviral construct that has both Thy1.1 and RFP.

Vector map:

Cellecta-pRSI16Thy-U6-sh-HTS6-UbiC-TagRFP-2A-Thy1.1-Map



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