American Society of Human Genetics (ASHG) Annual Meeting

October 15-19, 2019, George R. Brown Convention Center, Houston, TX

Stop by and see us at Booth 1007.

Poster Presentation: Wednesday, October 16, 2019, 2:00 to 3:00 pm

Poster 1875W: Clonal analysis, genetic screening, and expression profiling at a single-cell level

Presenter: Alex Chenchik, PhD, President & CSO, Cellecta

Co-Lab Presentation: Thursday, October 17, 2019, 2:30 to 3:00 pm at Exhibit Hall, Booth 1209

Single-Cell Functional Genomics to Study Tumor Development
Novel methods for profiling clonal composition and phenotypes of primary and metastatic lesions at the single-cell level are required for understanding tumorigenesis mechanisms and development of anti-cancer drug with unique mechanism of action. In order to facilitate these studies, we developed a panel of lentiviral barcoded libraries to label and monitor the cancer cells in a time course experiments in vitro and in mouse xenograft models. The barcodes are transcribed from lentiviral constructs and could be detected by NGS in a single-cell RNA expression profiling assay to identify the subpopulation of descendant cells derived from a single barcoded progenitor cell. Furthermore, cell barcodes were incorporated in conjunction with genetic effector libraries, such as sgRNA libraries, to identify clonal phenotypic changes induced by specific genetic disruptions in progeny cells derived from the single progenitor cell. However, while barcoding cells provides an effective way to group cells based on clonal origin in heterogenous cell populations, expression profiling at a single-cell level remains challenging. Data will be presented showing how genetic screen and targeted RNA expression profiling of human cells combined with cell barcoding could significantly improve phenotyping of distinct cell populations.

Presenter: Alex Chenchik, PhD, President & CSO, Cellecta

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