CRISPR 2.0 Next Generation Human Genome Editing Congress

November 17 - November 19, 2020 | Virtual Event

Functional CRISPR Screening and Cell Barcoding to identify genes driving biological responses and disease progression

Presenter: Paul Diehl, Ph.D., COO, Cellecta

Synopsis:

  • Pooled lentiviral libraries of CRISPR sgRNA have proven to be a highly effective approach to identify genes, and other genetic elements, driving biological responses
  • Libraries containing unique barcode sequences enable cell-specific labeling of individual cells in a target population to identify sub-populations that express specific features or phenotypes (e.g., pathway activation, drug resistance)
  •  Barcoded CRISPR guide library combined with single cell expression profiling enables parallel analysis of differences in gene activation across and within cell populations as a result of perturbation or selection

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