CloneTracker™ XP-CRISPR Human 27 Anti-Cancer CRISPR-Barcode-3 Library - RFP-Puro

Human sgRNA libraries incorporating clonal barcodes adjacent to the gRNA sequences. The library is designed for CRISP-Seq (aka Perturb-Seq) screens. In cells transduced with these libraries, researchers can detect both the clonal barcodes and the sgRNA effector with RNA Sequencing so that changes in gene expression can be correlated with specific disruptions of particular genes.

Cellecta currently offers two CloneTracker XP CRISPR Knockout libraries in the pRSGScribe1cb-EFS-TagRFP-2A-Puro-U6-sgRNA-BC30-3LTR vector, one targeting 27 human anti-cancer genes and one targeting the 27 corresponding mouse homologs. There are 20 sgRNAs to each gene designed to target functional domains.

Manual and Product Documentation

• Gene List: CloneTracker XP-CRISPR Human 27 Barcode-3' Library
• Manual: CRISPR Pooled Lentiviral sgRNA Libraries Manual
• References:
• https://doi.org/10.1016/j.cell.2016.11.038
• https://doi.org/10.1101/083774

Library Vector Information

* Sequences are available in FASTA (.txt), GenBank (.gb), and SnapGene (.dna) formats. You may use the free SnapGene Viewer to open .dna files. If you would like vector files in another format, please contact us. To download .txt and .gb files, right click on the link and Save As. 

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Alignment Software Supporting Files

• Library Configuration File (JSON format, compressed): Human 27 Anti-Cancer CRISPR-Barcode-3
• Sample Description Input Form: XLSM (Please enable macros)