Loss-of-Function RNAi Screen in PDX Mouse Models

May 27, 2016

Using a Cellecta-made shRNA library, groups from the European Institute of Oncology and MD Anderson Cancer Center published the first in vivo pooled RNAi screen in patient-derived tumor xenograft PDX mouse models. The study published in June Cancer Discovery screened 236 epigenetic genes targeted with a pooled shRNA...
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Millions of Defined Sequenceable Barcodes for Clonal Cell Tracking

February 26, 2014

The ability to identify and quantify the size of clonal cell populations produced by each cell in a founder population offers researchers a powerful tool for understanding how groups cells grow and proliferate in all sorts of conditions. This sort of clonal cell tracking enables cell biologists,...
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Researchers Identify SALL1 as a Tumor Suppressor in Breast Cancer with a Role in E-Cadherin Regulation

January 17, 2014

Researchers at the German Cancer Research Center (DKFZ) and the MD Anderson Cancer Center used Cellecta's shRNA DECIPHER Libraries for an in vivo genetic screen that identified SALL1 as a breast cancer tumor suppressor that plays a role in E-cadherin regulation (Article)E-cadherin (CDH1) has been shown to have a...
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RNAi Screening with An Inducible Promoter: Is There an Advantage?

May 13, 2011

Inducible expression is often desirable for functional genetic testing to establish clear cause and effect for a specific phenotype. A particular phenotype can be demonstrably linked to expression of a specific cDNA by showing it disappears when transcription is suppressed. Although less direct, similar logic...
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