Catalog #: KOHGW-80Kv2-P
Cellecta’s Single-module Human Genome-Wide CRISPR Knockout Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide "dropout viability" screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA tracrRNA structure which we have proven generates more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results.
If you are interested in more than 4 guides per gene, we offer a second Human Genome-Wide CRISPR Library (Knockout-B Library) which has an additional set of 4 guides per gene that do not overlap with the main library. Please contact us for more details.