How to Make a Crappy CRISPR Library

June 01, 2017

In addition to the pooled CRISPR libraries we offer, there are a few other libraries that researchers can choose to use for gene knockout screens, such as the Broad Institute’s GeCKO and Brunello libraries available through Addgene. To conserve the limited stock of these libraries, many labs only distribute a...
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A New GFP CRISPR for Primary and Non-Dividing Cells

April 29, 2015

Use of antibiotic resistance markers with CRISPR is an effective tool for cells that proliferate.  However, for primary cells and non-dividing cells, all-in-one sgRNA-Cas9 lentiviral vector that contains a fluorescence marker (GFP-CRISPR) could be a better option.Our standard, Single-Vector CRISPR-Cas9 System...
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CRISPR Knockout Screens with Optimal Cas9 to sgRNA Ratios

March 12, 2015

The previous blog post " Two-Vector CRISPR System Is Better Approach for Knockout Screens" discussed the advantages of expressing the Cas9 nuclease on a vector separate from the one used for the guide RNAs (sgRNA)—a two-vector CRISPR system—when using the system for complex pooled loss-of-function screens.  In...
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Two-Vector CRISPR System Is Better Approach for Knockout Screens

November 26, 2014

Since our introduction of the All-in-One CRISPR-Cas9 lentiviral system in the Spring, we have done a bit more work and moved forward with a two-vector system for our pooled libraries of sgRNA expression constructs. If the All-in-One CRISPR-Cas9 vector works well—which it does—why, you might wonder, would we...
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CRISPR Knockout Rate Dependent on Cas9 Expression Levels

May 28, 2014

Cellecta recently developed a one-plasmid CRISPR lentiviral vector that expresses both the sgRNA and Cas9 protein. CRISPR enables convenient of a target gene at the genomic level, as opposed to shRNA which knocks down the level of the RNA transcript for the target gene.Due to the differences between the shRNA...
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